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Extrachromosomal circular nuclear rDNA in Euglena gracilis.

P Ravel-Chapuis, P Nicolas, V Nigon

    Nucleic Acids Research
    |October 25, 1985
    PubMed
    Summary

    Extrachromosomal nuclear ribosomal DNA (rDNA) is abundant in Euglena gracilis, primarily existing as circular units. Its quantity fluctuates with growth phases, being higher during exponential growth.

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    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2014

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Genetics

    Background:

    • Nuclear ribosomal DNA (rDNA) plays a crucial role in ribosome biogenesis.
    • The localization of rDNA (chromosomal vs. extrachromosomal) can impact gene expression and regulation.
    • Previous research on extrachromosomal rDNA in other organisms like spinach and Petunia yielded negative results.

    Purpose of the Study:

    • To establish and characterize the presence of extrachromosomal nuclear ribosomal DNA (rDNA) in Euglena gracilis.
    • To investigate the physical characteristics and abundance of extrachromosomal rDNA in Euglena gracilis.
    • To determine the prevalence of extrachromosomal rDNA across different Euglena gracilis strains.

    Main Methods:

    • Microscopy and molecular techniques were employed to detect and analyze extrachromosomal rDNA.
    • Quantification of rDNA was performed during different growth phases (exponential and stationary).
    • Various wild-type and mutant strains of Euglena gracilis were examined.

    Main Results:

    • Extrachromosomal nuclear ribosomal DNA (rDNA) was confirmed in Euglena gracilis, predominantly as circular molecules.
    • Each circular rDNA unit measured 3.8 microns and contained a single rDNA unit, with rare oligomers observed.
    • Extrachromosomal rDNA levels were significantly higher during the exponential growth phase compared to the stationary phase and were present in all examined strains.

    Conclusions:

    • The majority of rDNA in Euglena gracilis exists in an extrachromosomal form.
    • Extrachromosomal rDNA is a stable feature across diverse Euglena gracilis strains.
    • The abundance of extrachromosomal rDNA is linked to the organism's growth dynamics.

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