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Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Related Experiment Video

Updated: Jun 20, 2026

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

Published on: October 18, 2010

Salmonella Detection in Food Using a HEK-hTLR5 Reporter Cell-Based Sensor.

Esma Eser^1,2, Victoria A Felton³, Rishi Drolia^1,3,4,5

¹Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.

|September 27, 2024

Summary

A novel cell-based biosensor effectively detects viable Salmonella in food within 15 hours. This rapid, sensitive method uses a human embryonic kidney (HEK293) cell line to identify live pathogens, enhancing food safety.

Keywords:

HEK-dual hTLR 5 Salmonella cell-based sensor detection flagella food safety immunomagnetic separation

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Last Updated: Jun 20, 2026

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

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Published on: October 18, 2010

Detection of Foodborne Bacterial Pathogens from Individual Filth Flies

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Published on: February 13, 2015

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Published on: November 7, 2018

Area of Science:

Food Microbiology
Biosensor Technology
Public Health

Background:

Rapid detection of foodborne pathogens like Salmonella is crucial for public health.
Existing methods face challenges in distinguishing live from dead cells and determining pathogenic potential.
Mammalian cell-based assays offer a way to detect live pathogens and active toxins.

Purpose of the Study:

To develop and validate a rapid, sensitive, and specific cell-based assay for detecting viable Salmonella in food.
To assess the performance of a human embryonic kidney (HEK293) cell line expressing Toll-Like Receptor 5 (TLR-5) as a biosensor.
To determine the total sample-to-result time for the developed assay.

Main Methods:

Utilized a HEK293 cell line engineered with TLR-5 and a chromogenic reporter system (HEK Dual hTLR5).
The assay detects flagellin, a TLR5 agonist, triggering a quantifiable alkaline phosphatase signal.
Specificity was tested against 20 Salmonella enterica serovars and 19 non-Salmonella species; performance was validated with spiked food samples.

Main Results:

The HEK Dual hTLR5 assay demonstrated specificity against a wide range of Salmonella serovars and non-Salmonella species.
The assay successfully detected viable Salmonella in various spiked food matrices (chicken, peanut butter, etc.).
A total detection time of 15 hours was achieved, including 8 hours of enrichment (4h pre-enrichment, 4h selective enrichment) for samples inoculated at 1-100 CFU/25 g.

Conclusions:

The HEK Dual hTLR5 cell-based functional biosensor shows significant potential for rapid screening of viable Salmonella in food.
This method addresses limitations of traditional assays by responding only to live pathogens.
The 15-hour sample-to-result time represents a substantial advancement in food safety testing.