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Induction of human stem cells into ameloblasts by reaggregation strategy.
Chensheng Lin1, Shiyu Liu2, Minjun Huang2
1Fujian Key Laboratory of Developmental and Neural Biology & Southern Center for Biomedical Research, College of Life Sciences, Fujian Normal University, Fuzhou, 350117, Fujian, P.R. China. linchensheng@fjnu.edu.cn.
Stem Cell Research & Therapy
|September 28, 2024
Summary
A new cell reaggregation method simplifies tooth regeneration, achieving over 90% success in tooth formation and 100% in ameloblast differentiation using human stem cells and mouse dental cells.
Area of Science:
- Stem cell biology
- Developmental biology
- Regenerative medicine
Background:
- Human stem cells, including induced pluripotent stem cells (hiPSCs), show potential for tooth regeneration.
- Current methods for generating bioengineered teeth face technical challenges and low success rates.
Purpose of the Study:
- To develop a simplified and effective strategy for inducing stem cells to form teeth and differentiate into ameloblasts.
- To establish a novel approach for creating stem cell-based tooth organoids and bioengineered tooth germs.
Main Methods:
- Directly reaggregating suspended human keratinocyte stem cells (hKSCs) or hiPSCs with mouse dental mesenchymal cells (mDMCs).
- Assessing tooth formation and ameloblastic differentiation rates after subrenal culture.
- Analyzing stem cell differentiation pathways using GFP tracing, RT-PCR, and histological analysis.
Main Results:
- Achieved >90% success in tooth formation and 100% in ameloblastic differentiation with 1-10% hKSCs or 5% hiPSCs.
- Observed hiPSCs differentiate into epidermal cells before dental epithelial cells upon interaction with mDMCs.
- Epithelial-derived hiPSCs demonstrated superior tooth formation potential compared to mesenchymal-derived hiPSCs.
Conclusions:
- A simplified cell-cell reaggregation strategy effectively induces stem cells for tooth formation and ameloblast differentiation.
- This method offers a promising pathway for developing in vitro tooth organoids and bioengineered tooth germs.
