Related Experiment Video
Updated: Jun 11, 2025

Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice
Published on: June 29, 2018
Study of the Synchronization and Transmission of Intracellular Signaling Oscillations in Cells Using Bispectral
Maxim E Astashev1,2, Dmitriy A Serov1,2, Arina V Tankanag2
1Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilove St. 38, 119991 Moscow, Russia.
Abstract:
The oscillation synchronization analysis in biological systems will expand our knowledge about the response of living systems to changes in environmental conditions. This knowledge can be used in medicine (diagnosis, therapy, monitoring) and agriculture (increasing productivity, resistance to adverse effects). Currently, the search is underway for an informative, accurate and sensitive method for analyzing the synchronization of oscillatory processes in cell biology. It is especially pronounced in analyzing the concentration oscillations of intracellular signaling molecules in electrically nonexcitable cells. The bispectral analysis method could be applied to assess the characteristics of synchronized oscillations of intracellular mediators. We chose endothelial cells from mouse microvessels as model cells. Concentrations of well-studied calcium and nitric oxide (NO) were selected for study in control conditions and well-described stress: heating to 40 °C and hyperglycemia. The bispectral analysis allows us to accurately evaluate the proportion of synchronized cells, their synchronization degree, and the amplitude and frequency of synchronized calcium and NO oscillations. Heating to 40 °C increased cell synchronization for calcium but decreased for NO oscillations. Hyperglycemia abolished this effect. Heating to 40 °C changed the frequencies and increased the amplitudes of synchronized oscillations of calcium concentration and the NO synthesis rate. The first part of this paper describes the principles of the bispectral analysis method and equations and modifications of the method we propose. In the second part of this paper, specific examples of the application of bispectral analysis to assess the synchronization of living cells in vitro are presented. The discussion compares the capabilities of bispectral analysis with other analytical methods in this field.
Related Concept Videos
Reconstruction of Signal using Interpolation
Aliasing
If the sampling frequency is below the Nyquist rate, these replicas overlap, preventing the original...
Graphical and Analytic Representation of Sinusoids
The first step is measuring the peak-to-peak value, which is twice the amplitude of the sinusoid. This provides information about the maximum voltage swing of the waveform.
Secondly, the period and angular frequency are determined. The period is the time taken for one complete cycle of the waveform, while...
Forced Oscillations
Circadian Rhythms and Gene Regulation
Bandpass Sampling
A bandpass signal has a spectrum with a lower frequency limit, denoted as ω1, and an upper frequency limit, denoted as ω2....

