Developing a Microsatellite Polymerase Chain Reaction System for Small Yellow Croaker (Larimichthys polyactis) and...

Area of Science:

• Aquaculture
• Genetics
• Conservation Biology

Background:

• The small yellow croaker (Bahaba taishanensis) is an economically vital fish in East Asia facing population decline.
• Overfishing and environmental changes threaten small yellow croaker populations.
• Effective breeding programs are crucial for species recovery, requiring accurate parentage assignment.

Purpose of the Study:

• To develop and validate a multiplex PCR system for precise parentage assignment in small yellow croaker.
• To support genetic improvement and sustainable aquaculture practices for the species.

Main Methods:

• Utilized the small yellow croaker reference genome to identify polymorphic microsatellite markers.
• Optimized a multiplex PCR system for simultaneous amplification of selected markers.
• Validated system accuracy with controlled and group mating trials.

Main Results:

• The multiplex PCR system achieved high accuracy in assigning parentage for offspring.
• The system proved effective in both controlled and group mating scenarios.
• Demonstrated reliability for pedigree management and selective breeding applications.

Conclusions:

• The developed multiplex PCR system is a valuable tool for small yellow croaker breeding programs.
• Facilitates pedigree management, selective breeding, and conservation efforts.
• Supports sustainable aquaculture and genetic improvement of the small yellow croaker.