Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

88
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
88

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis of Carboxymethyl Dextran-Coated Gold Nanoparticles as Stable and Storable Optical Labels for Ultrasensitive Plasmonic Nanoparticle-Linked Sorbent Assay.

Sensors (Basel, Switzerland)·2025
Same author

Practical and Compact Guided Mode Resonance Sensing System for Highly Sensitive Real-Time Detection.

Sensors (Basel, Switzerland)·2025
Same author

Integration of CRISPR/Cas12a and a Fiber Optic Particle Plasmon Resonance Sensor for Single Nucleotide Polymorphism Detection in an Aldehyde Dehydrogenase 2 Gene.

ACS sensors·2025
Same author

Label-Free Biosensor Based on Particle Plasmon Resonance Coupled with Diffraction Grating Waveguide.

Sensors (Basel, Switzerland)·2024
Same author

Noninvasive Prenatal Genetic Screening of Cell-Free Fetal DNA for Early Prediction of β-Thalassemia Using Fiber Optic Nanogold-Linked Sorbent Assay.

ACS sensors·2024
Same author

Theoretical Analysis of GeSn Quantum Dots for Photodetection Applications.

Sensors (Basel, Switzerland)·2024
Same journal

Correction: Kang et al. Fluid Flow to Electricity: Capturing Flow-Induced Vibrations with Micro-Electromechanical-System-Based Piezoelectric Energy Harvester. <i>Micromachines</i> 2024, <i>15</i>, 581.

Micromachines·2026
Same journal

Femtosecond Laser Texturing of Wood Coatings with Bio-Based Epoxy and Wax Additives for Enhanced Hydrophobicity.

Micromachines·2026
Same journal

Engineering of Optoelectronic Devices for Renewable Energy Applications.

Micromachines·2026
Same journal

Phase Transformation and Electrochemical Behavior of Hexagonal TiO<sub>2</sub> Nanotubes Under Different Annealing Temperatures and Heating Rates.

Micromachines·2026
Same journal

Process Optimization and Predictive Modeling of Femtosecond Laser Precision Milling for Commercial PMMA Slices.

Micromachines·2026
Same journal

A Hybrid Preprocessing Multi-Objective Surrogate Model for Thermal MEMS Actuators.

Micromachines·2026
See all related articles

Related Experiment Video

Updated: May 3, 2026

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
09:04

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Published on: September 17, 2017

7.6K

Waveguide-Enhanced Nanoplasmonic Biosensor for Ultrasensitive and Rapid DNA Detection.

Devesh Barshilia1, Akhil Chandrakanth Komaram2, Lai-Kwan Chau2

  • 1Department of Mechanical Engineering and Advanced Institute of Manufacturing with High-Tech Innovations, National Chung Cheng University, Chiayi 621301, Taiwan.

Micromachines
|September 28, 2024
PubMed
Summary
This summary is machine-generated.

A new DNA biosensor uses gold nanoparticles and waveguides for rapid, enzyme-free detection. This ultrasensitive, cost-effective technology offers a promising tool for early disease diagnosis and clinical applications.

Keywords:
DNAgold nanoparticleoptical biosensorplanar waveguidereal time detection

More Related Videos

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.6K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.2K

Related Experiment Videos

Last Updated: May 3, 2026

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
09:04

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Published on: September 17, 2017

7.6K
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.6K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.2K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Molecular Biology

Background:

  • DNA and RNA sequence analysis is crucial for diagnostics, gene expression monitoring, and pathogen detection.
  • Traditional methods like PCR and NGS are complex, costly, and require advanced computational skills.
  • There is a need for rapid, low-cost, enzyme-free, and amplification-free DNA detection strategies.

Purpose of the Study:

  • To develop a novel DNA-functionalized waveguide-enhanced nanoplasmonic optofluidic biosensor.
  • To achieve enzyme-free and amplification-free DNA detection with high sensitivity and speed.
  • To create a cost-effective and clinically applicable DNA detection platform.

Main Methods:

  • Fabrication of a biosensor integrating gold nanoparticles (AuNPs) with a glass planar waveguide (WG) and microfluidic channel using vacuum-free methods.
  • Development of a nanogold-linked sorbent assay with a sandwich architecture for specific DNA target detection.
  • Utilizing AuNPs labeled with DNA probes to enhance sensitivity via evanescent wave manipulation and plasmon resonance.

Main Results:

  • The biosensor achieved specific detection of complementary DNA sequences.
  • Demonstrated an ultrasensitive limit of detection (LOD) of 33.1 fg/mL (4.36 fM).
  • Exhibited a rapid response time of approximately 8 minutes with minimal nonspecific adsorption.

Conclusions:

  • The developed biosensor is ultrasensitive, rapid, cost-effective, and suitable for clinical applications.
  • The innovative design and fabrication are advantageous for mass production.
  • This technology presents a viable tool for precise disease diagnostics and improved clinical outcomes.