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Host Factor Rab4b Promotes Japanese Encephalitis Virus Replication
Qin Zhao1,2,3, Chang Miao1, Yi-Ting Chen1
1Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Microorganisms
|September 28, 2024
Summary
Rab4b protein is crucial for Japanese encephalitis virus (JEV) replication. Knocking out Rab4b inhibits JEV, while overexpressing it promotes viral proliferation, impacting adsorption, internalization, assembly, and release.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Japanese encephalitis virus (JEV) infects various cell types, but its host receptors remain unclear.
- Previous studies identified Rab4b as a potential host factor for JEV infection in PK15 cells using CRISPR/Cas9 screening.
Purpose of the Study:
- To investigate the role of Rab4b in the JEV life cycle.
- To elucidate how Rab4b influences JEV replication in PK15 cells.
Main Methods:
- Utilized CRISPR/Cas9 technology to create Rab4b knockout and overexpression PK15 cell lines.
- Employed immunofluorescence assay (IFA), RT-qPCR, and Western blot to analyze JEV replication.
- Studied the impact of Rab4b on JEV adsorption, internalization, assembly, and release.
Main Results:
- Rab4b knockout significantly inhibited JEV replication in PK15 cells.
- Rab4b overexpression promoted JEV replication in PK15 cell lines.
- Demonstrated Rab4b's role in facilitating JEV adsorption, internalization, assembly, and release.
Conclusions:
- Host factor Rab4b plays a critical role in promoting JEV replication throughout its entire life cycle.
- This study enhances understanding of the JEV-host factor interaction network.
- Provides a foundation for further research into Rab4b protein function in viral infections.
