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Performance Comparison of Four Hepatitis E Antibodies Detection Methods
Milagros Muñoz-Chimeno1, Nazaret Díaz-Sánchez1, Lucía Morago1
1Hepatitis Unit, National Center of Microbiology, Instituto de Salud Carlos III, 28220 Madrid, Spain.
Microorganisms
|September 28, 2024
Summary
This study compared four Hepatitis E virus (HEV) antibody detection methods. LIAISON® MUREX demonstrated superior sensitivity for detecting acute HEV IgM infections and accurate IgG results compared to competitors.
Area of Science:
- Virology
- Immunology
- Diagnostic Medicine
Background:
- Hepatitis E virus (HEV) antibody detection is crucial for diagnosing HEV infection.
- Evaluating the performance of different diagnostic assays is essential for accurate and timely diagnosis.
Purpose of the Study:
- To compare the sensitivity and specificity of four HEV antibody detection techniques: LIAISON® MUREX, VIRCLIA®, WANTAI, and VIDAS®.
- To identify the most effective assay for detecting both acute and convalescent HEV infections.
Main Methods:
- Utilized five sample panels configured according to immunoblot standards.
- Assessed anti-HEV IgM and IgG detection using LIAISON® MUREX, VIRCLIA®, WANTAI, and VIDAS® assays.
Main Results:
- All techniques showed 100% sensitivity for anti-HEV IgM in the acute phase.
- LIAISON® MUREX exhibited higher sensitivity (96.74%) in acute and early convalescent phases compared to VIRCLIA® (83.14%), WANTAI (84.78%), and VIDAS® (88.04%).
- LIAISON® MUREX and VIRCLIA® achieved 100% specificity for anti-HEV IgM, while LIAISON® showed higher IgG specificity (97.17%) than VIRCLIA® (88.68%).
Conclusions:
- LIAISON® MUREX anti-HEV IgM assay offers superior performance in detecting acute HEV infections.
- The LIAISON® MUREX assay provides accurate anti-HEV IgG results, making it a reliable choice for HEV diagnosis.
