Magnetic Nanoparticles with On-Site Azide and Alkyne Functionalized Polymer Coating in a Single Step through a...

Area of Science:

• Materials Science
• Nanotechnology
• Biomedical Engineering

Background:

• Magnetic iron oxide nanoparticles (MNPs) are increasingly vital in various scientific fields.
• MNPs were synthesized via a one-step solvothermal method, utilizing azide and alkyne functionalized polyethylene glycol (PEG) polymers and β-cyclodextrin (βCD) as stabilizers.

Purpose of the Study:

• To develop and characterize novel magnetic nanoparticles with tailored surface functionalities.
• To evaluate their magnetic properties, stability, and potential for biomedical applications, including drug conjugation and hyperthermia.

Main Methods:

• Solvothermal synthesis of MNPs with PEG and βCD coatings.
• Characterization using TEM, AFM, NMR, XRD, FT-IR, MALDI, and VSM.
• Evaluation of magnetic properties, specific absorption rate (SAR) under alternating magnetic field (AMF), click chemistry conjugation, and in vitro cytotoxicity assays.

Main Results:

• Synthesized nanoparticles (@Fe3O4-PEGs and @Fe3O4-βCD) ranged from 90-250 nm in diameter.
• MNPs exhibited superparamagnetism with high saturation magnetization (up to 59.9 emu/g) and no ferromagnetism.
• Demonstrated successful click chemistry conjugation with a cannabidiol derivative and showed excellent dispersibility in aqueous media for over two weeks.
• Achieved SAR values up to 51.87 ± 2.23 W/g and exhibited no toxicity in the T84 human colon cancer cell line.

Conclusions:

• The developed magnetic nanoparticles possess desirable superparamagnetic properties, excellent stability, and biocompatibility.
• Their susceptibility to click chemistry and demonstrated non-toxicity make them highly promising for diverse biomedical applications, including targeted drug delivery and cancer hyperthermia therapy.