Related Experiment Video
Updated: Jun 11, 2025

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
Published on: August 31, 2017
Unveiling the Connection: Viral Infections and Genes in dNTP Metabolism
Shih-Yen Lo1,2, Meng-Jiun Lai1, Chee-Hing Yang1,3
1Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien 970, Taiwan.
Abstract:
Deoxynucleoside triphosphates (dNTPs) are crucial for the replication and maintenance of genomic information within cells. The balance of the dNTP pool involves several cellular enzymes, including dihydrofolate reductase (DHFR), ribonucleotide reductase (RNR), and SAM and HD domain-containing protein 1 (SAMHD1), among others. DHFR is vital for the de novo synthesis of purines and deoxythymidine monophosphate, which are necessary for DNA synthesis. SAMHD1, a ubiquitously expressed deoxynucleotide triphosphohydrolase, converts dNTPs into deoxynucleosides and inorganic triphosphates. This process counteracts the de novo dNTP synthesis primarily carried out by RNR and cellular deoxynucleoside kinases, which are most active during the S phase of the cell cycle. The intracellular levels of dNTPs can influence various viral infections. This review provides a concise summary of the interactions between different viruses and the genes involved in dNTP metabolism.
Related Concept Videos
Viral Mutations
Viral Recombination
Retroviruses
Mechanisms of Retrovirus-induced Cancers
Retrovirus Life Cycles
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon...

