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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples
Published on: March 28, 2015
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Short Communication: Rotavirus Group A Occurrence in Rural Water Source Samples in a Midwest Region State of Brazil,
Graziela Picciola Bordoni1, Lucas Candido Gonçalves Barbosa2, Fernando Santos Lima1
1Institute of Tropical Pathology and Public Health, Federal University of Goiás, 235 Street, Goiânia 74605-050, Brazil.
Viruses
|September 28, 2024
Summary
Rotavirus (RV) poses a waterborne disease risk. This study found RV in rural Goiás water sources was significantly more prevalent during the dry season compared to the rainy season.
Area of Science:
- Environmental microbiology
- Public health virology
- Water quality assessment
Background:
- Rotavirus (RV) is a primary cause of waterborne diseases and recognized by WHO as a potential reference pathogen.
- Contaminated water sources pose significant public health risks, leading to outbreaks and epidemics.
Purpose of the Study:
- To detect and correlate the presence of Rotavirus in rural water sources in Goiás.
- To investigate the seasonal prevalence of Rotavirus in drinking water during dry and rainy periods.
Main Methods:
- Collection of 86 water samples during the dry season and 160 during the rainy season from rural Goiás.
- Concentration of water samples, extraction of viral genetic material, and application of molecular testing.
- Statistical analysis to determine the association between Rotavirus presence and season.
Main Results:
- Rotavirus was detected in 54.7% of samples during the dry season and 20% during the rainy season.
- A strong statistical association (p-value < 0.001) was found between Rotavirus presence and the dry season.
- The dry season showed significantly higher Rotavirus prevalence in the studied water sources.
Conclusions:
- Rotavirus contamination in rural water sources in Goiás is significantly associated with the dry season.
- Findings highlight the need for enhanced water safety measures, particularly during dry periods, to mitigate risks of waterborne diseases.
- Seasonal monitoring of Rotavirus in drinking water is crucial for public health protection in endemic areas.
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