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Identification and detection of conserved G-quadruplex in monkeypox virus using conformation specific fluorogenic

Sumon Pratihar¹, Ramjayakumar Venkatesh¹, Mohamed Nabeel Mattath¹

¹Bioorganic Chemistry Laboratory, New Chemistry Unit, Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR), Jakkur, P.O., Bengaluru, 560064 Karnataka, India. tgraju@jncasr.ac.in.

Chemical Communications (Cambridge, England)

|September 30, 2024

Summary

Researchers found stable G-quadruplex (GQ) structures in monkeypox virus (MPV) DNA, paving the way for new diagnostic tools. A novel fluorogenic probe method was developed to detect these specific MPV G-quadruplex structures.

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Area of Science:

Genomics
Structural Biology
Virology

Background:

Noncanonical DNA structures play roles in various biological processes.
Identifying pathogen-specific DNA structures is key for developing targeted diagnostics.
Monkeypox virus (MPV) poses a significant public health threat.

Purpose of the Study:

To identify and characterize novel noncanonical DNA structures within the MPV genome.
To investigate the stability and conservation of these structures.
To develop a sensitive detection method for these structures.

Main Methods:

Bioinformatic analysis of conserved MPV DNA sequences.
In silico prediction and experimental validation of G-quadruplex (GQ) structures.
Development and application of a fluorogenic probe assay for GQ detection.

Main Results:

Stable G-quadruplex (GQ) structures were identified in conserved DNA regions unique to the MPV genome.
These GQ structures were found to be stable under physiological conditions.
A sensitive fluorogenic probe-based method for detecting MPV-specific GQ structures was successfully developed.

Conclusions:

Conserved G-quadruplex structures represent a unique genomic feature of the monkeypox virus.
These findings provide a foundation for developing novel MPV diagnostic strategies.
The developed detection method offers a promising approach for rapid and specific MPV identification.