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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jun 11, 2025

Quantification of Circular RNAs Using Digital Droplet PCR

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

Analytical Methods to Evaluate RNA Circularization Efficiency.

Yali Sun¹, Anis H Khimani¹, Yanhong Tong¹

¹Revvity Health Sciences, Revvity, Inc., Waltham, Massachusetts, USA.

Electrophoresis

|September 30, 2024

Summary

This summary is machine-generated.

This study introduces new analytical methods for evaluating synthetic circular RNA (circRNA) production. These methods aid researchers in optimizing circRNA vector engineering and understanding ribozyme catalytic activity for RNA therapeutics.

Keywords:

circular RNA linear RNA microfluidic capillary electrophoresis vector engineering

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Last Updated: Jun 11, 2025

Quantification of Circular RNAs Using Digital Droplet PCR

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Published on: July 29, 2019

Area of Science:

Biochemistry and Molecular Biology
RNA Therapeutics
Synthetic Biology

Background:

Circular RNAs (circRNAs) are crucial in RNA therapeutics due to their stable, closed-loop structure.
Ribozyme-based strategies are common for synthetic circRNA production.
Current analytical methods lack high-throughput and quantitative capabilities for circRNA vector engineering.

Purpose of the Study:

To develop and detail analytical methods for characterizing ribozyme-based RNA circularization.
To enable high-throughput and quantitative evaluation of circRNA synthesis efficiency.
To support optimization of circRNA vector engineering and ribozyme catalytic activity.

Main Methods:

Detailed description of analytical methodologies for circRNA characterization.
Evaluation of ribozyme-based RNA circularization efficiency.
Focus on quantitative and high-throughput analysis.

Main Results:

Established robust analytical methods for assessing circRNA circularization.
Provided a framework for quantitative evaluation of synthetic circRNA production.
Demonstrated utility in optimizing ribozyme-mediated circularization.

Conclusions:

The developed analytical methods are essential for advancing synthetic circRNA production.
These methods facilitate efficient circRNA vector engineering and optimization.
The study contributes to the field of RNA therapeutics by improving synthetic RNA production analysis.