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Importance of a reference ladder in analytical testing of CRISPR HDR donor ssDNA templates

Zhi-Xiang Lu1, Anis H Khimani1, Yanhong Tong1

  • 1Revvity, Inc, Waltham, MA, USA.

Biotechniques
|December 5, 2024
PubMed
Abstract

No abstract available in PubMed .

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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