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Related Experiment Video

Updated: Jun 11, 2025

Dissection of Hippocampal Dentate Gyrus from Adult Mouse
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Transcriptomic Profiles of AKAP12 Deficiency in Mouse Corpus Callosum.

Tomonori Hoshino1, Hajime Takase1,2, Hidehiro Ishikawa1,3

  • 1Neuroprotection Research Laboratories, Departments of Radiology and Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA.

Bioinformatics and Biology Insights
|September 30, 2024
PubMed
Summary

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This summary is machine-generated.

A-kinase anchor protein 12 (AKAP12) deficiency minimally impacts corpus callosum gene expression. Downregulation of BBB-related genes Klf2 and Sgk1 suggests potential roles in neurological disorders.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genomics

Background:

  • A-kinase anchor protein 12 (AKAP12) is a scaffold protein involved in central nervous system functions, including blood-brain barrier (BBB) regulation.
  • AKAP12 exhibits higher expression in the corpus callosum compared to other brain regions, yet its specific role there is unknown.

Purpose of the Study:

  • To investigate the functional impact of AKAP12 deficiency in the corpus callosum.
  • To identify genes and pathways affected by AKAP12 loss in this brain region.

Main Methods:

  • Transcriptome analysis using RNA-sequencing (RNA-seq).
  • Comparison of gene expression profiles in AKAP12 knockout (KO) mice versus wild-type controls.

Main Results:

Keywords:
AKAP12RNA-seqcorpus callosumknockout mice

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  • Minimal transcriptional changes were observed in the corpus callosum of AKAP12 KO mice, with only 13 differentially expressed genes.
  • Genes Klf2 and Sgk1, potentially involved in BBB function, were significantly downregulated.
  • Klf2 and Sgk1 expression patterns in vascular cells mirrored that of Akap12.

Conclusions:

  • AKAP12 deficiency has a limited direct effect on corpus callosum gene expression.
  • Downregulation of Klf2 and Sgk1 suggests a potential role for AKAP12 in regulating BBB-associated pathways.
  • Findings provide a dataset for further research into AKAP12's CNS functions and neurological disorder implications.