Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exposure to Endocrine-disrupting Chemicals, Iron Metabolism, and Risk of Metabolic Dysfunction-associated Steatotic Liver Disease: A Nationwide Cross-sectional Study in China.

Biomedical and environmental sciences : BES·2026
Same author

[Dietary sodium intake and food sources among adult residents in 16 provinces (autonomous regions and municipalities) of China from 2022-2024].

Wei sheng yan jiu = Journal of hygiene research·2026
Same author

Ultrasensitive In Vivo Imaging of Adoptive Immune Cell Distribution and Expansion Using Second Near-Infrared Conjugated Oligoelectrolyte Probes.

Research (Washington, D.C.)·2026
Same author

Adaptive genomic evolution and WD40-regulated temporal dynamics of anthocyanins support leaf photoplasticity in Parrotia subaequalis.

BMC plant biology·2026
Same author

<i>CDHR2</i> c.2233C > T Is Involved in Human Familial Ovarian Immature Teratoma With <i>BMP15</i> c.262C > T.

Human mutation·2026
Same author

Mechanical Properties and Microscopic Mechanism of Shield Tunnel Spoil Stabilized with ESCA.

Materials (Basel, Switzerland)·2026

Related Experiment Video

Updated: Jun 11, 2025

Mouse Adipose Tissue Collection and Processing for RNA Analysis
11:13

Mouse Adipose Tissue Collection and Processing for RNA Analysis

Published on: January 31, 2018

48.6K

Optimizing total RNA extraction method for human and mice samples.

Yumei Zeng1, Xiaoxue Tang2, Jinwen Chen3

  • 1Department of Neurology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China.

Peerj
|September 30, 2024
PubMed
Summary
This summary is machine-generated.

A modified TRIzol method using guanidine isothiocyanate (GITC-T) offers superior RNA extraction from human and mouse tissues. This cost-effective approach yields higher quality RNA, making it ideal for small-scale labs and advanced molecular studies.

Keywords:
GITC-T methodSDS-T methodTRIzol reagentThe TRIzol methodTotal RNA extraction method

More Related Videos

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |
10:06

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Published on: November 13, 2011

15.7K
A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
00:13

A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia

Published on: September 21, 2019

5.6K

Related Experiment Videos

Last Updated: Jun 11, 2025

Mouse Adipose Tissue Collection and Processing for RNA Analysis
11:13

Mouse Adipose Tissue Collection and Processing for RNA Analysis

Published on: January 31, 2018

48.6K
Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |
10:06

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Published on: November 13, 2011

15.7K
A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
00:13

A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia

Published on: September 21, 2019

5.6K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • High-quality total RNA extraction is crucial for advanced molecular studies like Next-generation sequencing.
  • Traditional methods (e.g., TRIzol, CTAB) can be complex and costly for small laboratories.
  • There is a need for practical, economical RNA extraction methods that maintain high efficiency and quality for human and mouse tissues.

Purpose of the Study:

  • To enhance the TRIzol method for improved RNA extraction efficiency and cost-effectiveness.
  • To evaluate modified TRIzol methods, specifically incorporating guanidine isothiocyanate (GITC-T) and sodium dodecyl sulfate (SDS-T).
  • To optimize RNA extraction from human and mouse tissues for small-scale laboratory applications.

Main Methods:

  • Modified TRIzol methods incorporating guanidine isothiocyanate (GITC-T) and sodium dodecyl sulfate (SDS-T) were developed.
  • RNA quality and yield were assessed using micro-volume UV-visible spectrophotometry, electrophoresis, quantitative PCR (q-PCR), and RNA sequencing (RNA-Seq).
  • Comparative analysis was performed against the standard TRIzol reagent method.

Main Results:

  • The GITC-T method demonstrated higher RNA yields, integrity, and purity compared to the standard TRIzol method.
  • Spectrophotometry showed improved OD260/280 and OD260/230 ratios for RNA extracted via GITC-T.
  • q-PCR and RNA-Seq confirmed superior transcript abundance and overall RNA quality using the GITC-T method from mouse cerebral cortex tissue.

Conclusions:

  • The GITC-T method provides a more efficient and cost-effective alternative for total RNA extraction.
  • This enhanced method yields higher purity and quantity of RNA, with reduced reagent consumption.
  • The GITC-T method is a feasible and practical option for small-scale laboratories and diverse RNA-based research.