Polypyrrole as Adsorbent in Magnetic Solid Phase Extraction for Progesterone Determination from Human Plasma

Area of Science:

• Analytical Chemistry
• Materials Science

Background:

• Progesterone is crucial for human development and fertility.
• Accurate quantification of progesterone in human plasma is essential.

Purpose of the Study:

• To develop a straightforward and effective chromatographic method for progesterone analysis.
• To utilize a novel polypyrrole/magnetic nanoparticle composite for sample preparation.

Main Methods:

• Magnetic solid-phase extraction with a polypyrrole/magnetic nanoparticle composite.
• High-performance liquid chromatography (HPLC) with an octadecyl silane column.
• Characterization of the composite using XRD, FTIR, SEM/EDS, TGA, and point of zero charge.

Main Results:

• High extraction recoveries (98%-102%) for progesterone.
• Excellent linearity (5-3000 ng mL⁻¹) with a correlation coefficient (r) ≥0.99.
• Successful analysis of progesterone in plasma samples from pregnant women, demonstrating method validation.

Conclusions:

• The developed method is effective and straightforward for progesterone analysis in human plasma.
• The polypyrrole/magnetic nanoparticle composite is a suitable adsorbent for magnetic solid-phase extraction.
• The validated method provides accurate and precise quantification of progesterone.