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Updated: Jun 11, 2025
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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
Published on: June 23, 2022
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Migratory autolysosome disposal mitigates lysosome damage
Takami Sho1, Ying Li2, Haifeng Jiao1
1State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Centre for Life Sciences, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing, China.
The Journal of Cell Biology
|September 30, 2024
Summary
Cells expel damaged lysosomes through a process called migratory autolysosome disposal (MAD). This mechanism requires cell migration and autophagy, aiding in organelle quality control and extracellular vesicle release.
Area of Science:
- Cell Biology
- Molecular Biology
- Organelle Biology
Background:
- Lysosomes are vital for cellular degradation and recycling.
- Cells utilize lysophagy and membrane repair for lysosome damage control.
- Maintaining lysosomal functionality is crucial for cellular homeostasis.
Purpose of the Study:
- To identify novel mechanisms of lysosome damage control.
- To investigate the role of cell migration in lysosomal repair.
- To understand the link between lysosome quality control and extracellular vesicle release.
Main Methods:
- Investigated cellular responses to lysosomal damage.
- Utilized techniques to track lysosome exocytosis.
- Analyzed the involvement of autophagy machinery and SNARE proteins.
Main Results:
- Discovered migratory autolysosome disposal (MAD) as a novel lysosome damage response.
- MAD involves the exocytosis of LAMP1-LC3 positive structures.
- This process requires autophagy, SNARE proteins, and cell migration.
Conclusions:
- Migratory autolysosome disposal (MAD) is a key mechanism for mitigating lysosomal damage.
- Cell migration plays a significant role in organelle quality control.
- Lysosome exocytosis facilitates the release of extracellular vesicles, linking cell migration and organelle health.
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