γδ T-Cell Immunophenotype for the Study of Human Aging
Anna Maria Corsale1,2, Marta Di Simone1,2, Serena Meraviglia1,2
1Central Laboratory of Advanced Diagnosis and Biomedical Research, University Hospital "P. Giaccone", Palermo, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|September 30, 2024
Abstract:
Flow cytometry serves as a crucial tool in immunology, allowing for the detailed analysis of immune cell populations. γδ T cells, a subset of T cells, play pivotal roles in immune surveillance and immune aging. Assessing the phenotype and functional capabilities of γδ T cells isolated from whole blood or tissue within the context of human aging yields invaluable insights into the dynamic changes affecting immune function, tissue homeostasis, susceptibility to infections, and inflammatory responses.
Keywords:
Cell expansionDensity gradient centrifugationFlow cytometryFunctional assaysImmunomagnetic separationTumor-infiltrating lymphocytesγδ T cells

