Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel mouse model of chronic Pseudomonas aeruginosa infection inducing bronchiectasis-like phenotype.

Animal models and experimental medicine·2026
Same author

The natural isoflavone puerarin mitigates cerebral ischemia-reperfusion injury by directly targeting CaMKⅡδ to block DLG4-Ser654 phosphorylation.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

[Retracted] Combined application of Embelin and tumor necrosis factor-related apoptosis-inducing ligand inhibits proliferation and invasion in osteosarcoma cells via caspase-induced apoptosis.

Oncology letters·2026
Same author

Molecular Mechanisms and Metabolic Responses in the Biological Antagonism Between <i>Trichoderma harzianum</i> and <i>Fusarium oxysporum</i>.

Microorganisms·2026
Same author

Case Report: Anti-PD-1 therapy as a catalyst for the rapid transformation of a hepatic nodule into HCC: a "soil and seed" paradox in metachronous triple primary malignancies?

Frontiers in immunology·2026
Same author

Integrating single-cell and spatial transcriptomics reveals glycolysis heterogeneity and NEK6-mediated progression in colorectal cancer.

Frontiers in immunology·2026

Related Experiment Video

Updated: May 11, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
00:09

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

21.7K

Exploring retinal degenerative diseases through CRISPR-based screening.

Rui Li1,2, Fengming Yang1,2, Boling Chu1,2

  • 1Sichuan Provincial Key Laboratory for Human Disease Gene Study and the Center for Medical Genetics, Department of Laboratory Medicine, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Molecular Biology Reports
|September 30, 2024
PubMed
Summary

CRISPR screening offers a powerful new approach for understanding retinal degenerative diseases. This review explores CRISPR tools, screening methods, and their application to advance retinal research and develop novel therapies.

Keywords:
CRISPR ScreeningCRISPR/Cas9Gene editingRetinal degenerative diseases

More Related Videos

Subretinal Transplantation of Human Embryonic Stem Cell-Derived Retinal Tissue in a Feline Large Animal Model
07:43

Subretinal Transplantation of Human Embryonic Stem Cell-Derived Retinal Tissue in a Feline Large Animal Model

Published on: August 5, 2021

1.9K
Author Spotlight: Exploring Retinal Regeneration Mechanisms in the Xenopus Frog
09:29

Author Spotlight: Exploring Retinal Regeneration Mechanisms in the Xenopus Frog

Published on: October 13, 2023

1.4K

Related Experiment Videos

Last Updated: May 11, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
00:09

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

21.7K
Subretinal Transplantation of Human Embryonic Stem Cell-Derived Retinal Tissue in a Feline Large Animal Model
07:43

Subretinal Transplantation of Human Embryonic Stem Cell-Derived Retinal Tissue in a Feline Large Animal Model

Published on: August 5, 2021

1.9K
Author Spotlight: Exploring Retinal Regeneration Mechanisms in the Xenopus Frog
09:29

Author Spotlight: Exploring Retinal Regeneration Mechanisms in the Xenopus Frog

Published on: October 13, 2023

1.4K

Area of Science:

  • Genetics and Molecular Biology
  • Ophthalmology
  • Biotechnology

Background:

  • The CRISPR/Cas9 system is a revolutionary gene-editing technology with broad applications.
  • CRISPR screening has advanced fields like cancer biology and drug discovery.
  • Retinal degenerative diseases urgently require new treatments due to poorly understood mechanisms.

Purpose of the Study:

  • To provide a comprehensive review of CRISPR tools and screening methodologies.
  • To explore the potential of CRISPR screening in retinal degenerative disease research.
  • To guide experimental design and identify research questions for retinal applications.

Main Methods:

  • Review of CRISPR/Cas9 system evolution and current screening platforms.
  • Discussion of experimental design, including selection strategies and delivery methods for CRISPR components (sgRNAs, Cas proteins).
  • Exploration of suitable in vitro models for retinal CRISPR screening.

Main Results:

  • CRISPR screening has significant, yet largely untapped, potential in retinal research.
  • Pioneering studies and experimental guidelines for CRISPR screening in retinal contexts are presented.
  • Identification of key research questions addressable by CRISPR screening in retinal diseases.

Conclusions:

  • This review highlights the transformative potential of CRISPR screening for retinal degenerative disorders.
  • It aims to foster innovation in understanding retinal pathophysiology.
  • The findings are expected to accelerate therapeutic development and improve patient outcomes.