Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phosphate determination by enzymatic colorimetric assay.

P Fossati

    Analytical Biochemistry
    |August 15, 1985
    PubMed
    Summary

    This study introduces a new colorimetric assay for measuring inorganic phosphate in serum. The method is fast, accurate, and suitable for automation, offering reliable results for clinical diagnostics.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A stratified treatment algorithm in psychiatry: a program on stratified pharmacogenomics in severe mental illness (Psych-STRATA): concept, objectives and methodologies of a multidisciplinary project funded by Horizon Europe.

    European archives of psychiatry and clinical neuroscience·2024
    Same author

    Patients' needs in proton therapy: A survey among ten European facilities.

    Clinical and translational radiation oncology·2023
    Same author

    [A guide to psychiatric prescribing in pregnancy, postpartum and breastfeeding].

    L'Encephale·2023
    Same author

    [Belief updating and mood congruence in depressive disorder].

    L'Encephale·2021
    Same author

    [Anticholinergic scales: Use in psychiatry and update of the anticholinergic impregnation scale].

    L'Encephale·2021
    Same author

    Psychotropics and COVID-19: An analysis of safety and prophylaxis.

    L'Encephale·2021

    Area of Science:

    • Clinical Chemistry
    • Biochemical Assays
    • Enzymology

    Background:

    • Accurate measurement of inorganic phosphate in serum is crucial for diagnosing various metabolic disorders.
    • Existing methods may have limitations in speed, sensitivity, or automation compatibility.

    Purpose of the Study:

    • To develop and validate a direct, rapid, and sensitive colorimetric assay for serum inorganic phosphate.
    • To utilize enzymatic reactions and a specific chromogen for accurate phosphate quantification.

    Main Methods:

    • Employs purine-nucleoside phosphorylase and xanthine oxidase to generate superoxide ions.
    • Measures superoxide using 3-(4',5'-dimethyl-2-thiazolyl)-2,4-diphenyl-2H-tetrazolium bromide chromogen.
    • Optimized for a 1:100 sample/reagent ratio, room temperature reaction, and 15-minute completion time.

    Main Results:

    • Linear standard curve for inorganic phosphate up to 4.9 mmol/liter.
    • Average analytical recovery of 100% in human sera.
    • Within-run precision with coefficient of variation (CV) ≤ 1.0%.

    Conclusions:

    • The developed colorimetric assay provides a reliable and efficient method for serum inorganic phosphate determination.
    • High correlation (r > 0.99) with the established semidine method validates its accuracy.
    • The assay's characteristics make it well-suited for automated laboratory workflows.

    Related Experiment Videos