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Diosmetin Promotes Early Embryonic Development in Pigs by Alleviating Oxidative Stress.
Jia-Jun Ren1, Xiu-Wen Yuan1, Zi-Long Meng1
1Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, South China Institute of Large Animal Models for Biomedicine, Wuyi University, Jiangmen, China.
Molecular Reproduction and Development
|October 1, 2024
Summary
Diosmetin (DIOS) enhances early pig embryonic development by reducing oxidative stress and apoptosis. Supplementation improved blastocyst rates and cell numbers, indicating a protective effect on embryo quality.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Biochemistry
Background:
- Diosmetin (DIOS), a flavonoid, possesses known antioxidant and anti-inflammatory properties.
- The effects of DIOS on early porcine embryonic development are currently unknown.
- Understanding factors that improve in vitro embryo development is crucial for swine reproduction.
Purpose of the Study:
- To investigate the impact of DIOS supplementation on porcine embryo development in vitro.
- To elucidate the molecular mechanisms by which DIOS influences embryonic development, focusing on oxidative stress, apoptosis, and mitochondrial function.
Main Methods:
- Porcine embryos were cultured in vitro with varying concentrations of DIOS.
- Assessed key developmental parameters: blastocyst formation rate and total cell number.
- Quantified levels of reactive oxygen species (ROS), glutathione (GSH), and mitochondrial membrane potential.
- Analyzed the expression of genes related to apoptosis, autophagy, embryonic development, and antioxidant defense.
Main Results:
- DIOS supplementation (0.1 μM) significantly increased blastocyst formation rate and total cell number.
- DIOS reduced ROS levels, decreased apoptosis markers (CASPASE3), and lowered autophagy markers (LC3B).
- DIOS enhanced antioxidant capacity by increasing Nrf2 and GSH levels and improving mitochondrial membrane potential.
Conclusions:
- Diosmetin promotes early porcine embryonic development by mitigating oxidative stress and enhancing mitochondrial function.
- DIOS exerts its beneficial effects by downregulating apoptosis and autophagy pathways.
- DIOS shows potential as a supplement to improve the quality of in vitro-produced porcine embryos.
