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Related Experiment Video

Updated: May 13, 2026

Subtype-selective Electroporation of Cortical Interneurons

Subtype-selective Electroporation of Cortical Interneurons

Published on: August 18, 2014

Transcriptomic cell-type specificity of local cortical circuits.

Maribel Patiño¹, Marley A Rossa², Willian Nuñez Lagos³

¹Systems Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA; Medical Scientist Training Program, University of California, San Diego, La Jolla, CA, USA.

|October 1, 2024

Summary

This summary is machine-generated.

Researchers developed a new method to map brain connections. This technique reveals specific input rules between transcriptomic subtypes of inhibitory and excitatory neurons in the mouse visual cortex.

Keywords:

cell subtypes cortical circuits cortical connectivity interneurons laminar specificity monosynaptic rabies tracing rabies tracing single-nuclei RNA sequencing transcriptomics visual cortex

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Last Updated: May 13, 2026

Subtype-selective Electroporation of Cortical Interneurons

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Published on: August 18, 2014

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

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Published on: June 20, 2018

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

Area of Science:

Neuroscience
Molecular Biology
Genomics

Background:

Neocortical functions depend on complex excitatory and inhibitory neuron networks.
Existing methods lack resolution to define connectivity rules at the transcriptomic subtype level.

Purpose of the Study:

To develop and apply a novel method for mapping synaptic inputs to transcriptomic cell types.
To characterize the transcriptomic subtypes of inhibitory neurons that synapse onto specific excitatory neuron populations in the mouse visual cortex.

Main Methods:

Single transcriptome assisted rabies tracing (START): combines monosynaptic rabies tracing with single-nuclei RNA sequencing.
Application of START to identify inhibitory inputs to 5 layer-specific excitatory neuron populations in mouse primary visual cortex (V1).

Main Results:

Confirmed known connectivity patterns at the neuronal subclass level.
Demonstrated transcriptomic subtype-specific inhibitory inputs to excitatory neuron subclasses.
Established precise connectivity rules at the resolution of transcriptomic inhibitory cell types.

Conclusions:

START provides unprecedented resolution for mapping neuronal connectivity.
Inhibitory neuron connectivity is highly specific at the transcriptomic subtype level.
These findings advance our understanding of local circuit organization in the neocortex.