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A plasmonic biosensor pre-diagnostic tool for Familial Mediterranean Fever
Idil Karaca Acari1, Fatma Kurul2, Meryem Beyza Avci2
1Department of Engineering Basic Sciences, Faculty of Engineering and Natural Sciences, Malatya Turgut Ozal University, Yesilyurt, Malatya, Turkey.
Abstract:
Familial Mediterranean Fever (FMF) is an autosomal recessive genetic disorder, primarily observed in populations around the Mediterranean Sea, linked to MEFV gene mutations. These mutations disrupt inflammatory responses, increasing pyrin-protein production. Traditional diagnosis relies on clinical symptoms, family history, acute phase reactants, and excluding similar syndromes with MEFV testing, which is expensive and often inconclusive due to heterozygous mutations. Here, we present a biosensor platform that detects differences in pyrin-protein levels between healthy and affected individuals, offering a cost-effective alternative to genetic testing. Our platform uses gold nanoparticle-based plasmonic chips enhanced with anti-pyrin antibodies, achieving a detection limit of 0.24 ng/mL with high specificity. The system integrates an optofluidic system and visible light spectroscopy for real-time analysis, with signal stability maintained for up to six months. Our technology will enhance FMF diagnosis accuracy, enabling early treatment initiation and providing a cost-effective alternative to genetic testing, thus improving patient care.
Insights
A new biosensor platform detects pyrin protein levels for diagnosing Familial Mediterranean Fever (FMF), offering a cost-effective alternative to expensive genetic testing for improved patient care.
Area of Science:
- Biomedical Engineering
- Molecular Diagnostics
- Genetic Disorders
Background:
- Familial Mediterranean Fever (FMF) is an autosomal recessive genetic disorder linked to MEFV gene mutations.
- Current diagnostic methods rely on clinical symptoms, family history, and MEFV gene testing, which can be costly and inconclusive.
- Accurate and early diagnosis of FMF is crucial for timely treatment and improved patient outcomes.
Purpose of the Study:
- To develop and validate a novel biosensor platform for detecting pyrin protein levels in FMF diagnosis.
- To provide a cost-effective and accurate alternative to traditional genetic testing for FMF.
- To enhance early diagnosis and treatment initiation for FMF patients.
Main Methods:
- Utilized gold nanoparticle-based plasmonic chips functionalized with anti-pyrin antibodies.
- Integrated an optofluidic system with visible light spectroscopy for real-time pyrin protein detection.
- Assessed the biosensor's detection limit, specificity, and signal stability.
Main Results:
- The biosensor achieved a low detection limit of 0.24 ng/mL with high specificity for pyrin protein.
- Demonstrated significant differences in pyrin protein levels between healthy individuals and FMF patients.
- The developed system exhibited signal stability for up to six months.
Conclusions:
- The novel biosensor platform offers a promising, cost-effective diagnostic tool for FMF.
- This technology can significantly improve diagnostic accuracy and facilitate earlier treatment for FMF.
- The biosensor represents a valuable advancement in managing Familial Mediterranean Fever.
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