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Sphingolipid Analysis in Clinical Research.

Jeongah Oh^1,2, Bo Burla³, Sneha Muralidharan¹

¹Singapore Lipidomics Incubator (SLING), Life Sciences Institute, National University of Singapore, Singapore, Singapore.

Methods in Molecular Biology (Clifton, N.J.)

|October 1, 2024

Summary

This summary is machine-generated.

This study details sphingolipid analysis in clinical research, offering methods for experimental design, sample handling, and measurement via liquid chromatography-mass spectrometry for understanding disease-related lipid alterations.

Keywords:

Ceramide Clinical mass spectrometry Glucosylceramide Lipidomics Mass spectrometry Quality control Sphingolipidomics Sphingolipids Sphingomyelin Sphingosine-1-phosphate

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Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Area of Science:

Biochemistry
Molecular Biology
Clinical Research

Background:

Sphingolipids are a diverse lipid class crucial for cell membranes and signaling.
Sphingolipid metabolism alterations are implicated in various human pathologies.
Lipidomic analysis is key to identifying these disease-associated changes.

Purpose of the Study:

To provide a guide for designing sphingolipidomic experiments in clinical research.
To outline proper handling procedures for plasma and serum samples.
To detail the measurement of sphingolipids using liquid chromatography-mass spectrometry.

Main Methods:

Experimental design considerations for sphingolipidomic studies.
Standardized protocols for plasma and serum sample preparation.
Liquid chromatography-mass spectrometry (LC-MS) for sphingolipid quantification.

Main Results:

Established methodologies for robust sphingolipid profiling.
Identified critical steps for accurate sample handling and analysis.
Demonstrated the utility of LC-MS in detecting disease-relevant sphingolipid changes.

Conclusions:

Properly designed lipidomic experiments are essential for clinical research.
Standardized methods ensure reliable sphingolipid measurements.
This approach aids in understanding sphingolipid roles in human diseases.