Related Experiment Video
Updated: Jun 11, 2025

Targeted DNA Methylation Analysis by Next-generation Sequencing
Published on: February 24, 2015
DNAMarkMaker: streamlining ARMS and CAPS marker development from resequencing data with NGS short reads
Tenta Segawa1, Sorachi Saiga1, Marina Takata1
1Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
DNAMarkMaker automates DNA marker development for Amplification Refractory Mutation System (ARMS) and Cleaved Amplified Polymorphic Sequences (CAPS) markers. This tool accelerates genetic analysis and crop breeding by simplifying primer design.
Area of Science:
- Plant genetics
- Bioinformatics
- Molecular breeding
Background:
- DNA markers are crucial for crop breeding and genetic analysis.
- Developing DNA markers is often time-consuming and labor-intensive, requiring polymorphism identification and primer design.
Purpose of the Study:
- To develop DNAMarkMaker, an automated tool for designing primers for ARMS and CAPS DNA markers.
- To streamline the DNA marker development process using resequencing data.
Main Methods:
- Developed DNAMarkMaker with a user-friendly graphical user interface (GUI).
- Utilized resequencing data for automated primer design.
- Tested the tool on rice, potato, and turnip with diverse genome structures.
Main Results:
- DNAMarkMaker successfully automated primer design for ARMS and CAPS markers.
- The tool demonstrated applicability across different plant species and ploidy levels.
- Facilitated marker development for homozygous diploid, heterozygous autotetraploid, and heterozygous diploid genomes.
Conclusions:
- DNAMarkMaker significantly accelerates the development of DNA markers.
- The tool enhances efficiency in crop breeding and genetic analysis.
- DNAMarkMaker is accessible to researchers with varying bioinformatics expertise.
More Related Videos
13:24Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
Published on: April 11, 2016
06:40G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
Published on: March 22, 2018
Related Concept Videos
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Sanger Sequencing