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Updated: Aug 3, 2026
Preparation of Naringenin Solution for In Vivo Application
Published on: August 10, 2021
Cytotoxic Impact of Naringenin-Loaded Solid Lipid Nanoparticles on RIN5F Pancreatic β Cells via Autophagy Blockage
Pardis Mohammadi Pour1, Zeinab Nouri2, Dariush Ghasemi3
1Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Background:
Autophagy plays a crucial role in modulating the proliferation of cancer diseases. However, the application of Naringenin (Nar), a compound with potential benefits against these diseases, has been limited due to its poor solubility and bioavailability.
Objective:
This study aimed to develop solid lipid nanoparticles (Nar-SLNs) loaded with Nar to enhance their therapeutic impact.
Methods:
In vitro experiments using Rin-5F cells exposed to Nar and Nar-SLNs were carried out to investigate the protective effects of Nar and its nanoformulation against the pancreatic cancer cell line of Rin-5F.
Results:
Treatment with Nar and Nar-SLN led to an increase in autophagic markers (Akt, LC3, Beclin1, and ATG genes) and a decrease in the level of miR-21. Both Nar and Nar-SLN treatments inhibited cell proliferation and reduced the expression of autophagic markers. Notably, Nar-SLNs exhibited greater efficacy compared to free Nar.
Conclusion:
These findings suggest that SLNs effectively enhance the cytotoxic impact of Nar, making Nar-SLNs a promising candidate for suppressing or preventing Rin-5F cell growth.
Insights
Naringenin solid lipid nanoparticles (Nar-SLNs) show enhanced efficacy in inhibiting pancreatic cancer cell growth by modulating autophagy. This nanoformulation improves the therapeutic potential of Naringenin (Nar) against cancer.
Area of Science:
- Cancer Research
- Nanotechnology
- Cell Biology
Background:
- Autophagy is critical in regulating cancer cell proliferation.
- Naringenin (Nar) shows anti-cancer potential but is limited by poor solubility and bioavailability.
- Developing effective delivery systems for Nar is crucial for cancer therapy.
Purpose of the Study:
- To develop Naringenin-loaded solid lipid nanoparticles (Nar-SLNs).
- To enhance the therapeutic efficacy of Nar against pancreatic cancer cells.
- To investigate the effects of Nar-SLNs on autophagy and cell proliferation.
Main Methods:
- In vitro experiments using Rin-5F pancreatic cancer cells.
- Exposure of cells to free Naringenin (Nar) and Naringenin-loaded solid lipid nanoparticles (Nar-SLNs).
- Analysis of autophagic markers (Akt, LC3, Beclin1, ATG genes) and miR-21 levels.
Main Results:
- Both Nar and Nar-SLNs increased autophagic markers and decreased miR-21.
- Nar-SLN treatment demonstrated greater inhibition of cell proliferation compared to free Nar.
- Nar-SLNs showed a more significant reduction in autophagic marker expression.
Conclusions:
- Solid lipid nanoparticles (SLNs) effectively enhance the cytotoxic effects of Naringenin (Nar).
- Nar-SLNs represent a promising strategy for suppressing pancreatic cancer cell growth.
- This nanoformulation improves Nar's bioavailability and therapeutic impact.
