Related Experiment Video
Updated: Jun 11, 2025
Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate CDAP
Published on: June 14, 2021
Exploration of Daptomycin Adsorption by Polymethylmethacrylate Hemofilter In Vitro
Yoshinori Inano1, Kayoko Tsuchiya1, Ryota Kumano1
1Division of Pharmacy, Eastern Chiba Medical Center.
Abstract:
Blood purification therapy with cytokine-adsorbing hemofilters has been used to treat sepsis-associated hypercytokinemia. Polymethylmethacrylate (PMMA) hemofilters are frequently used for this purpose; however, adsorption and removal of teicoplanin, a therapeutic agent, have been reported. Similar concerns have been shared regarding daptomycin because its structure resembles that of teicoplanin; nevertheless, there have been no reported effects associated with daptomycin in this context. We studied the adsorption of daptomycin onto a PMMA hemofilter in vitro and investigated its adsorption onto hollow fiber membranes by adding cut PMMA membranes to a daptomycin solution. Additionally, the daptomycin solution was circulated in a dialysis circuit connected to a PMMA hemofilter, and changes in daptomycin content were examined. The daptomycin content decreased immediately after adding the hollow fiber membranes, similar to that observed for teicoplanin. The daptomycin content was lower than that of the standard reagent in the dialysis circuit model, reaching values below the measurement limit after 20 min. These results suggested that daptomycin was adsorbed and removed by the PMMA hemofilter. Encountering this effect during clinical use is plausible; therefore, daptomycin administration via a PMMA hemofilter should be avoided during blood purification therapy.
Insights
Polymethylmethacrylate (PMMA) hemofilters may adsorb and remove the antibiotic daptomycin during blood purification therapy. This interaction, similar to teicoplanin, could reduce daptomycin efficacy, necessitating caution during concurrent administration.
Area of Science:
- Nephrology
- Pharmacology
- Critical Care Medicine
Background:
- Blood purification therapy utilizes cytokine-adsorbing hemofilters to manage sepsis-associated hypercytokinemia.
- Polymethylmethacrylate (PMMA) hemofilters are common, but known to adsorb the antibiotic teicoplanin.
- Daptomycin shares structural similarities with teicoplanin, raising concerns about potential adsorption by PMMA hemofilters.
Purpose of the Study:
- To investigate the in vitro adsorption of daptomycin onto PMMA hemofilters.
- To assess the impact of PMMA hemofilters on daptomycin levels in a simulated dialysis circuit.
Main Methods:
- In vitro adsorption experiments using PMMA hollow fiber membranes and daptomycin solutions.
- Circulation of daptomycin solution through a dialysis circuit equipped with a PMMA hemofilter.
- Quantification of daptomycin levels before and after passage through the hemofilter.
Main Results:
- Daptomycin content significantly decreased upon contact with PMMA hollow fiber membranes.
- In the dialysis circuit model, daptomycin levels dropped below the measurement limit within 20 minutes.
- These findings indicate substantial adsorption and removal of daptomycin by PMMA hemofilters.
Conclusions:
- PMMA hemofilters adsorb and remove daptomycin, potentially impacting therapeutic efficacy.
- Clinical co-administration of daptomycin and PMMA hemofilter therapy should be avoided.
- Further investigation into the clinical implications of daptomycin adsorption during blood purification is warranted.
