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Related Concept Videos

Structure of Cadherins01:25

Structure of Cadherins

The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
Desmosomes01:05

Desmosomes

The term desmosome derives from the Greek words "desmo" and "soma" meaning "adhesion bodies." This structure was first observed during the late 1800s and described as small, dense nodules in the epidermis. Desmosomes are button-like structures that help form an interlinked network of intermediate filaments across the cells. These junctions are  essential to hold cells together under mechanical stress and to maintain tissue integrity. Desmosomes are multi-protein complexes comprising desmosomal...
Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Tight Junctions01:29

Tight Junctions

Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

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Related Experiment Video

Updated: Jun 18, 2026

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

Intestinal Tuft Cells Are Enriched With Protocadherins.

Rachel Stubler1, Sarah A Dooley1, Rachel Edens1

  • 1Department of Regenerative Medicine & Cell Biology, Medical University of South Carolina, Charleston, SC.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|October 3, 2024
PubMed
Summary

Intestinal tuft cells utilize protocadherins, specifically CDHR2 and CDHR5, to organize their unique apical microvilli. These findings reveal key structural components essential for tuft cell function in both mice and humans.

Keywords:
IMACepitheliumgutmicrovilli

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Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections
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Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections

Published on: March 21, 2018

Related Experiment Videos

Last Updated: Jun 18, 2026

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
09:51

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

Published on: December 13, 2017

Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections
06:26

Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections

Published on: March 21, 2018

Area of Science:

  • Gastroenterology and Cell Biology
  • Molecular and Cellular Biology
  • Immunology

Background:

  • Intestinal tuft cells are rare chemosensory cells crucial for immune signaling.
  • Their apical microvilli structure is vital for function but not fully understood.
  • Intermicrovillar adhesion complexes (IMACs) stabilize microvilli in other intestinal cells.

Purpose of the Study:

  • To investigate the role of IMACs in intestinal tuft cell microvillar organization.
  • To identify specific proteins involved in maintaining tuft cell structure.
  • To compare protein localization in murine and human tuft cells.

Main Methods:

  • Immunohistochemistry on murine and human intestinal tissues.
  • Colocalization studies with known tuft cell markers (DCLK1, phospho-EGFR, advillin, cytokeratin 18).
  • Single-cell RNA sequencing of human intestinal cells.

Main Results:

  • Cadherin-related family member-2 (CDHR2) and cadherin-related family member-5 (CDHR5) were found in tuft cells.
  • CDHR2 and CDHR5 colocalized with tuft cell markers in murine intestine.
  • Human single-cell data and immunostaining confirmed CDHR2 and CDHR5 presence and apical localization in human tuft cells.
  • Usher syndrome type 1 C (USH1C) and Myosin 7b were present but less abundant.

Conclusions:

  • Protocadherins, specifically CDHR2 and CDHR5, are essential components of intestinal tuft cells.
  • These proteins likely play a critical role in organizing the apical microvilli of tuft cells.
  • The findings are conserved across murine and human intestinal tuft cells.