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Structural isomerism engineering regulates molecular AIE behavior and application in visualizing endogenous hydrogen
Yaxi Li1, Yong-Xiang Wang2, Dujuan Liu1
1Department of Radiology, Jiangnan University Medical Center (JUMC), Wuxi 214002, China.
Journal of Materials Chemistry. B
|October 4, 2024
Summary
Researchers developed a novel coumarin-based fluorescent probe (CMR-o-Py) for sensitive, real-time detection of hydrogen sulfide (H2S). This probe shows promise for biological applications, including sensing endogenous H2S in living cells.
Area of Science:
- Chemical Biology
- Materials Science
- Biomedical Engineering
Background:
- Hydrogen sulfide (H2S) is a crucial signaling molecule in physiological and pathological processes.
- Accurate detection of H2S is vital for understanding its role in diseases like cancer and neurodegeneration.
- Fluorescence-based detection offers advantages for real-time, in situ H2S sensing.
Purpose of the Study:
- To develop a novel coumarin-based fluorescent probe for sensitive H2S detection.
- To investigate the aggregation-induced emission (AIE) properties of the synthesized fluorophores.
- To evaluate the probe's performance for sensing H2S in biological environments.
Main Methods:
- Synthesis of coumarin-based fluorophores linked with aminopyridine via an imino-double bond.
- Structural isomerism engineering to optimize AIE behavior.
- Characterization of probe performance, including detection limit, binding affinity, and reaction kinetics.
- Application of the probe in sensing endogenous H2S in living cells and onion tissues.
Main Results:
- Developed CMR-o-Py, a coumarin derivative exhibiting enhanced AIE properties.
- Achieved a low detection limit of 18.1 μM for H2S in a hydrophilic environment.
- Demonstrated a high binding energy constant (5.68 × 10^8 M^-1) and rapid binding kinetics.
- Confirmed excellent biocompatibility and successful sensing of endogenous H2S in biological samples.
Conclusions:
- CMR-o-Py nanoaggregates function as effective fluorescent probes for H2S detection.
- The probe's design allows for sensitive and selective sensing of H2S.
- CMR-o-Py shows significant potential for real-time monitoring of H2S in biological systems and disease research.
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