Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interleukin 2 and its cell-surface receptor.

R J Robb

    Behring Institute Mitteilungen
    |August 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Interleukin 2 (IL-2) signals immune cell growth and function through its receptor. High-affinity IL-2 receptor binding is crucial for physiological responses, potentially involving multiple receptor subunits.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Interleukin 2: the molecule and its function.

    Immunology today·2014
    Same author

    In situ vaccination against a non-immunogenic tumour using intratumoural injections of liposomal interleukin 2.

    Cytokine·2002
    Same author

    The effect of cholesterol in a liposomal Muc1 vaccine.

    Biochimica et biophysica acta·2001
    Same author

    Interleukin-2-induced small unilamellar vesicle coalescence.

    Biochimica et biophysica acta·2001
    Same author

    Biopharmaceutics of liposomal interleukin 2, oncolipin.

    Cytokine·2000
    Same author

    A comparison of biodistribution of liposomal and soluble IL-2 by a new method based on time-resolved fluorometry of europium.

    Cytokine·2000
    Same journal

    Clinical Experience with ALG.

    Behring Institute Mitteilungen·2010
    Same journal

    Production of a Standardized Anti-Lymphocyte Globulin.

    Behring Institute Mitteilungen·2010
    Same journal

    Immunosuppression and Neoplasia.

    Behring Institute Mitteilungen·2010
    Same journal

    Molecular and Immunological Aspects of Host-Parasite Interactions. Symposium proceedings. Wurzburg, Germany, June 6-8, 1996.

    Behring Institute Mitteilungen·1997
    Same journal

    The galactose-inhibitable surface lectin of Entamoeba histolytica, a possible candidate for a subunit vaccine to prevent amoebiasis.

    Behring Institute Mitteilungen·1997
    Same journal

    Plasmodium vivax: epitope mapping of monoclonal antibodies against the N-terminal region of the merozoite surface protein 1.

    Behring Institute Mitteilungen·1997
    See all related articles

    Area of Science:

    • Immunology
    • Molecular Biology
    • Cell Signaling

    Background:

    • Interleukin 2 (IL-2) is a critical lymphokine mediating immune cell proliferation and differentiation.
    • IL-2 function relies on interaction with a high-affinity cell surface receptor.
    • Structural integrity of IL-2, including a disulfide bridge and specific amino acid regions, is essential for its activity.

    Purpose of the Study:

    • To investigate the structural requirements for IL-2 activity.
    • To elucidate the characteristics of the IL-2 receptor and its binding affinity.
    • To understand the molecular basis of IL-2 mediated immune responses.

    Main Methods:

    • Antibody-mediated blocking assays to identify functional epitopes on IL-2.
    • Analysis of IL-2 receptor binding affinities (high vs. low).

    Related Experiment Videos

  • Expression of IL-2 receptor cDNA in cell lines to assess functional contribution.

    • Main Results:

    • Specific amino acid regions (8-27 and 33-54) of IL-2 are critical for blocking physiological responses.
    • Physiological responses correlate with high-affinity IL-2 receptor binding.
    • The IL-2 receptor protein alone does not mediate high-affinity binding, suggesting involvement of additional subunits.

    Conclusions:

    • The IL-2 receptor system involves multiple components for high-affinity ligand interaction.
    • Structural features of IL-2 are vital for receptor binding and biological activity.
    • The IL-2 system serves as a model for dissecting molecular immune responses.