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KRAS mutations detection methodology: from RFLP to CRISPR/Cas based methods.

Firouzeh Morshedzadeh1, Mohammad Reza Abbaszadegan2,3, Maryam Peymani4

  • 1Department of Genetics, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

Functional & Integrative Genomics
|October 4, 2024
PubMed
Summary

Identifying KRAS mutations is crucial for personalized cancer treatment. This review covers methods like digital droplet PCR, NGS, and PCR for detecting these mutations, aiding targeted therapy selection.

Keywords:
KRASMolecular diagnostic methodsPersonalized cancer medicineRAS family

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • KRAS mutations are key biomarkers in personalized cancer medicine.
  • Accurate identification of KRAS mutations guides treatment decisions and improves patient outcomes.
  • Understanding KRAS mutation status is vital for selecting appropriate targeted therapies.

Purpose of the Study:

  • To provide a comprehensive review of current KRAS mutation detection approaches.
  • To highlight the importance of rapid and reliable KRAS mutation discovery.
  • To guide clinicians in identifying KRAS mutations for effective cancer treatment.

Main Methods:

  • Review of established and emerging molecular testing techniques.
  • Discussion of methods including digital droplet PCR, next-generation sequencing (NGS), and polymerase chain reaction (PCR).
  • Analysis of challenges such as tumor heterogeneity and resistance mechanisms.

Main Results:

  • Various molecular methods offer effective KRAS mutation detection.
  • Fast and reliable detection facilitates personalized treatment strategies.
  • Understanding limitations is essential for accurate interpretation.

Conclusions:

  • KRAS mutation testing is indispensable for guiding targeted cancer therapy.
  • Continued advancements in detection methods are improving patient care.
  • This review serves as a clinical guide for KRAS mutation identification.