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Updated: Jun 11, 2025

Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

Trifluoperazine effect on human sperm: The accumulation of reactive oxygen species and the decrease in the

Houpeng Wang¹, Cheng Cheng², Jing Ding²

¹Jiangxi Provincial Key Laboratory of Disease Prevention and Public Health, Jiangxi Medical College, Nanchang University, Nanchang 330006, China; School of Public Health, Jiangxi Medical College, Nanchang University, China.

Reproductive Toxicology (Elmsford, N.Y.)

|October 6, 2024

Summary

Trifluoperazine (TFP), an antipsychotic, may harm male fertility. High TFP concentrations reduced sperm viability by increasing reactive oxygen species (ROS) and decreasing mitochondrial membrane potential (MMP).

Keywords:

Drug repurposing Human semen quality In vitro Intracellular calcium concentration Trifluoperazine

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Area of Science:

Reproductive Toxicology
Pharmacology

Background:

Antipsychotic drug use is linked to reduced human sperm quality.
Trifluoperazine (TFP), a common antipsychotic, is being investigated for anticancer properties.
The impact of TFP on human sperm quality is not well understood.

Purpose of the Study:

To investigate the effects of TFP on human sperm function and physiological parameters in vitro.
To determine the concentration-dependent toxicity of TFP on sperm.

Main Methods:

Human sperm were co-cultured with various concentrations of TFP (12.5, 25, 50, and 100 μM).
Sperm function and physiological parameters, including reactive oxygen species (ROS) production, mitochondrial membrane potential (MMP), viability, motility, penetration ability, capacitation, and acrosome reaction, were assessed.

Main Results:

TFP concentrations of 50 μM and 100 μM increased ROS and decreased MMP, leading to reduced sperm viability.
25 μM TFP inhibited sperm penetration ability, total motility, and progressive motility.
Lower TFP concentrations (12.5 and 25 μM) increased intracellular calcium ([Ca2+]i) but did not affect capacitation or acrosome reaction, possibly due to lack of increased tyrosine phosphorylation.

Conclusions:

TFP can induce male reproductive toxicity.
Mechanisms of toxicity include ROS accumulation and decreased MMP in human sperm.
These findings highlight potential risks of TFP use on male fertility.