Advanced applications in enzyme-induced electrospun nanofibers

Area of Science:

• Materials Science
• Biotechnology
• Nanotechnology

Background:

• Electrospun nanofibers possess high surface area, mechanical strength, and chemical versatility, making them ideal for enzyme immobilization.
• Enzyme-induced electrospun nanofibers (EIEN) have advanced significantly over the last decade.
• EIEN leverage various immobilization strategies for enhanced enzyme performance.

Purpose of the Study:

• To systematically review advanced applications of EIEN.
• To elucidate the rationale behind different EIEN immobilization methods.
• To provide guidelines for developing EIEN techniques for future applications.

Main Methods:

• Fabrication of EIEN using non-specific immobilization: interfacial adsorption (direct adsorption, cross-linking, covalent binding) and encapsulation.
• Fabrication of EIEN using specific immobilization techniques: coordination and affinity immobilization.
• Systematic review of EIEN applications in wastewater treatment, biosensors, and biomedicine.

Main Results:

• EIEN demonstrate broad applicability across environmental, sensing, and medical fields.
• Different immobilization methods offer distinct advantages for enzyme loading and stability.
• The review categorizes EIEN fabrication and application strategies.

Conclusions:

• Optimizing EIEN immobilization is crucial for balancing enzyme activity, stability, and cost-effectiveness.
• Further research is needed to facilitate the industrialization of EIEN.
• Tailoring EIEN immobilization techniques to specific applications is key for future development.