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Updated: Jun 11, 2025
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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
Published on: June 11, 2015
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Phage Hunting in the High School Classroom: Phage Isolation and Characterization.
Charles Bittle1, Kathryn Brittain2, Sarah M Doore3
1Lincoln Southwest High School.
The American Biology Teacher
|October 7, 2024
Summary
Students engage in hands-on science by hunting for bacteriophage, a type of virus, in environmental samples. This week-long activity involves sample collection, bacterial testing, and phage purification for scientific study.
Area of Science:
- Microbiology
- Molecular Biology
- Science Education
Background:
- National science education standards emphasize inquiry-based and hands-on learning experiences.
- Integrating authentic scientific practices into K-12 curricula is crucial for student engagement.
- Bacteriophage discovery offers a unique, accessible model for teaching fundamental biological concepts.
Purpose of the Study:
- To describe a week-long, hands-on laboratory activity for students to discover bacteriophage.
- To provide a framework for implementing authentic scientific research in an educational setting.
- To foster student understanding of microbial ecology and molecular techniques.
Main Methods:
- Students collected environmental samples (e.g., soil, water).
- Samples were screened for bacteriophage activity using a "bait" bacterial host.
- Isolated bacteriophage were amplified and purified for further characterization.
Main Results:
- Evidence of bacteriophage was detected in various environmental samples.
- Students successfully isolated and purified bacteriophage through a series of laboratory steps.
- The activity provided a tangible experience of the scientific discovery process.
Conclusions:
- This bacteriophage discovery curriculum effectively engages students in authentic scientific practices.
- The methodology is adaptable for different educational levels and resource settings.
- Hands-on research experiences enhance scientific literacy and inspire future scientists.
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