Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

3.4K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.4K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.3K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.3K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

6.2K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
6.2K
DNA Microarrays02:34

DNA Microarrays

17.2K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
17.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of Modified Atmosphere Packaging on Quality Maintenance of <i>Pleurotus pulmonarius</i> Under Simulated Logistics Temperature Fluctuations.

Foods (Basel, Switzerland)·2026
Same author

Separable syn/anti Bis-Boron Dyes with Stereochemistry-Dependent Packing and Aggregated-State Emission.

Organic letters·2026
Same author

Pyrolysis of heavy metal-enriched plant residues: stabilization of Pb/Zn/Cd and MnFe<sub>2</sub>O<sub>4</sub>-modified biochar for Congo red adsorption.

Environmental geochemistry and health·2026
Same author

The complete chloroplast genome of <i>Viola mandshurica</i> (Violaceae) and its phylogenetic analysis.

Mitochondrial DNA. Part B, Resources·2026
Same author

Computational advances in epigenetic regulation databases and prediction tools.

Biotechnology advances·2026
Same author

Astragalin from Morus alba L. ameliorates high-fat diet-induced obesity-related metabolic disturbances in mice and is associated with hepatic AMPK/PI3K/AKT signaling.

Journal of ethnopharmacology·2026

Related Experiment Video

Updated: Jun 11, 2025

Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer
08:20

Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer

Published on: May 21, 2019

5.6K

MolBiC: the cell-based landscape illustrating molecular bioactivities.

Yichao Ge1,2,3,4, Mengjie Yang5, Xinyuan Yu1

  • 1College of Pharmaceutical Sciences, The Second Affiliated Hospital, Zhejiang University School of Medicine, State Key Laboratory of Advanced Drug Delivery and Release Systems, Zhejiang University, Hangzhou 310058, China.

Nucleic Acids Research
|October 7, 2024
PubMed
Summary

A new database, MolBiC, offers over 550,000 cell-based molecular bioactivity (CMB) measurements. This resource supports AI-driven drug discovery by providing crucial data for learning cell-based patterns.

More Related Videos

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

3.2K
A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
12:04

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

9.6K

Related Experiment Videos

Last Updated: Jun 11, 2025

Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer
08:20

Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer

Published on: May 21, 2019

5.6K
Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

3.2K
A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
12:04

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

9.6K

Area of Science:

  • Biomedicine
  • Drug Discovery
  • Bioinformatics

Background:

  • Cell-based molecular bioactivity (CMB) is vital for drug development.
  • Existing resources lack comprehensive CMB data for AI-driven drug discovery.

Purpose of the Study:

  • Introduce MolBiC, a novel knowledge base for cellular context molecular bioactivity data.
  • Provide a valuable resource to facilitate AI-powered drug discovery and development.

Main Methods:

  • Compiled and curated a large dataset of experimentally validated CMBs.
  • Integrated data on molecules, targets, and cell lines.

Main Results:

  • MolBiC contains 550,093 experimentally validated CMBs.
  • The database includes 321,086 molecules, 2,666 targets, and 988 cell lines.
  • MolBiC is freely accessible online.

Conclusions:

  • MolBiC addresses the critical need for CMB data in modern drug discovery.
  • The database supports the learning of cell-based patterns for pharmaceutical development.
  • MolBiC is a unique and valuable resource for the scientific community.