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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Operando Freezing Cryogenic Electron Microscopy of Active Battery Materials.

Nikita S Dutta1, Gerard Michael Carroll1, Nathan R Neale1

  • 1Materials, Chemical, and Computational Science Directorate, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, USA.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|October 7, 2024
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This study introduces operando freezing cryo-electron microscopy (cryo-EM) to capture transient battery material structures during operation. This method preserves native states for advanced battery research.

Keywords:
operandobatteriescryogenic electron microscopyinterfaces

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Area of Science:

  • Materials Science
  • Electrochemistry
  • Microscopy

Background:

  • Understanding battery material evolution is key for safer, more efficient energy storage.
  • Cryogenic electron microscopy (cryo-EM) is valuable for battery characterization but sample prep can alter structures.
  • Current methods lack the ability to preserve dynamic changes occurring during battery cycling.

Purpose of the Study:

  • To develop and validate a method for operando freezing cryo-EM.
  • To preserve and characterize native electrode and interfacial structures during battery cycling.
  • To enable direct visualization of transient features in electrochemical systems.

Main Methods:

  • Development of an operando plunge freezer and cold sample removal process.
  • Application of the method to multiple electrode materials for validation.
  • Quantification and discussion of the achieved freezing rates.

Main Results:

  • Successful preservation of native electrode and interfacial structures during battery cycling.
  • Demonstration of operando freezing cryo-EM's capability to visualize transient features.
  • Validation of the method's effectiveness across various electrode materials.

Conclusions:

  • Operando freezing cryo-EM provides a powerful tool for studying dynamic processes in batteries.
  • This technique allows for a deeper understanding of structural evolution and interfacial chemistry.
  • Enables advancements in the design of next-generation energy storage systems.