Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

3.0K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
3.0K
Cytoplasm01:24

Cytoplasm

5.6K
The cytoplasm consists of organelles and a framework of protein scaffolds called the cytoskeleton suspended in an aqueous solution, the cytosol. The cytosol is a rich broth of water, ions, salts, and various organic molecules.
Protein Folding and Misfolding
The cytoplasm is the location for several cellular processes, including protein synthesis and folding. The aqueous nature of the cytosol promotes protein folding such that the hydrophobic amino acid side chains are buried in the protein...
5.6K
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

18.4K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
18.4K
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

1.8K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
1.8K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

5.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.7K
Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

7.0K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Special issue "Biophysics in the Iberian Peninsula".

Biophysical reviews·2026
Same author

Optimizing spatial organization of FtsZ rings for large-scale constriction in synthetic cells.

Nature communications·2026
Same author

Crosslinking by ZapD drives the assembly of short FtsZ filaments into toroidal structures in solution.

eLife·2025
Same author

Evidence for biomolecular condensates formed by the Escherichia coli MatP protein in spatiotemporal regulation of the bacterial cell division cycle.

International journal of biological macromolecules·2025
Same author

The effect of Ficoll 70 on thermally-induced and chemically-induced conformational transitions of an RTX protein is quantitatively accounted for by a unified excluded volume model.

Physical chemistry chemical physics : PCCP·2024
Same author

Evidence for biomolecular condensates of MatP in spatiotemporal regulation of the bacterial cell division cycle.

bioRxiv : the preprint server for biology·2024
Same journal

Peptideins: Navigating the gray zone of the proteome.

Trends in biochemical sciences·2026
Same journal

A metabolon channels nicotine biosynthesis.

Trends in biochemical sciences·2026
Same journal

Better call chaperone.

Trends in biochemical sciences·2026
Same journal

Biochemistry at scale: Seeing both the forest and the trees.

Trends in biochemical sciences·2026
Same journal

Voices across Asia and Oceania: Biochemistry across borders.

Trends in biochemical sciences·2026
Same journal

Metabolic control of RNA splicing by polyamines.

Trends in biochemical sciences·2026
See all related articles

Related Experiment Video

Updated: Jun 11, 2025

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
06:32

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Published on: July 28, 2022

2.2K

Surfaces as frameworks for intracellular organization.

Germán Rivas1, Allen P Minton2

  • 1CIB Margarita Salas - Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.

Trends in Biochemical Sciences
|October 7, 2024
PubMed
Summary
This summary is machine-generated.

Many soluble proteins reversibly bind to cell structures like fibers and membranes. This nonspecific attraction affects protein function and distribution within the cell.

Keywords:
cytoskeletal fibersnonspecific interactionsphospholipid membranesprotein adsorptionprotein associations

More Related Videos

Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits
10:32

Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits

Published on: April 15, 2015

8.5K
Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
09:12

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

Published on: June 9, 2022

5.5K

Related Experiment Videos

Last Updated: Jun 11, 2025

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
06:32

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Published on: July 28, 2022

2.2K
Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits
10:32

Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits

Published on: April 15, 2015

8.5K
Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
09:12

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

Published on: June 9, 2022

5.5K

Area of Science:

  • Cellular Biology
  • Biophysics

Background:

  • Soluble proteins within cells can reversibly associate with intracellular structures.
  • These structures include proteinaceous fibers and phospholipid membranes.

Purpose of the Study:

  • To present theoretical and experimental evidence for protein-surface associations.
  • To investigate the role of nonspecific attraction in these interactions.
  • To explore the consequences of these associations on protein function and cellular distribution.

Main Methods:

  • Theoretical modeling of protein-surface interactions.
  • Experimental validation of theoretical predictions.

Main Results:

  • Nonspecific attraction significantly contributes to protein association with cellular structures.
  • Adsorbed proteins exhibit altered association states.
  • Biological functions of proteins can be modified upon adsorption.
  • Protein distribution within cellular microenvironments is impacted.

Conclusions:

  • Nonspecific protein-surface interactions are a key factor in intracellular protein dynamics.
  • These interactions have profound implications for cellular organization and function.
  • Understanding these associations is crucial for comprehending cellular processes.