Calmodulin-binding proteins of Tetrahymena microsomal membranes

Tetrahymena calmodulin radioiodinated with a lactoperoxidase method retained full ability to activate Tetrahymena guanylate cyclase. Binding of [125I]calmodulin to Tetrahymena microsomal membranes was Ca2+-dependent and inhibited by excess unlabeled calmodulin or trifluoperazine. When Triton X-100-solubilized microsomes were chromatographed on calmodulin Sepharose, several proteins were found to interact with calmodulin in a Ca2+-dependent manner.