Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Emerging nano-delivery systems for targeting mitochondria.

Nanoscale·2025

Same author

Savinase-functionalised oxidative drug-loaded nanocarriers enhance the treatment of solid colorectal tumours in a 3D cell culture model.

Journal of materials chemistry. B·2025

Same author

Dual surface functionalised curcumin-shellac nano-delivery system with enhanced antimicrobial action.

RSC advances·2025

Same author

Enhanced anticancer effect of lysozyme-functionalized metformin-loaded shellac nanoparticles on a 3D cell model: role of the nanoparticle and payload concentrations.

Biomaterials science·2024

Same author

Stimulus triggered release of actives from composite microcapsules based on sporopollenin from <i>Lycopodium clavatum</i>.

RSC advances·2024

Same author

Natural sporopollenin microcapsules: biological evaluation and application in regulating hepatic toxicity of diclofenac sodium <i>in vivo</i>.

Biomaterials science·2023

Same journal

Melanocortin 1 receptor-targeted peptide-functionalized liposomes for enhanced melanocyte-preferential drug delivery and anti-melanogenic efficacy.

Journal of materials chemistry. B·2026

Same journal

Recent progress in side-chain amino acid-based polymers: synthesis, self-assembly, and emerging biomedical applications.

Journal of materials chemistry. B·2026

Same journal

Bioinspired electrospun nanofibrous dressings loaded with Mentha-derived exosome-like vesicles for antibacterial and immunomodulatory burn healing.

Journal of materials chemistry. B·2026

Same journal

On demand functionality of an NIR-enhanced nanozyme catalyst for infected wound healing.

Journal of materials chemistry. B·2026

Same journal

Positively charged, phenolic hydroxyl and anthraquinone structured polystyrene microspheres targeting dual elimination of bacterial pathogens and pathogen-associated molecular patterns for sepsis therapy.

Journal of materials chemistry. B·2026

Same journal

Carbon dot-decorated Ni-MOF heterojunction sonozymes for enhanced sonodynamic-chemodynamic cancer therapy.

Journal of materials chemistry. B·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 11, 2025

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Published on: March 26, 2013

3D structured capillary cell suspensions aided by aqueous two-phase systems.

Amro K F Dyab^1,2, Vesselin N Paunov¹

¹Department of Chemistry, Nazarbayev University, Kabanbay Batyr 53, Astana 010000, Kazakhstan. vesselin.paunov@nu.edu.kz.

Journal of Materials Chemistry. B

|October 8, 2024

Summary

This summary is machine-generated.

Researchers developed a simple method for 3D cell structuring using aqueous two-phase systems (ATPS). This technique creates unique 3D cell patterns in capillary bridges for applications in tissue engineering and cell culturing.

More Related Videos

Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing

Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing

Published on: April 23, 2015

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Related Experiment Videos

Last Updated: Jun 11, 2025

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Published on: March 26, 2013

Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing

Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing

Published on: April 23, 2015

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Area of Science:

Biotechnology
Cell Biology
Materials Science

Background:

3D cell structuring is crucial for tissue engineering and biomedical research.
Existing methods for cell patterning can be complex and limited.
Aqueous two-phase systems offer unique properties for manipulating biological materials.

Purpose of the Study:

To develop a facile technique for 3D structuring of living cells.
To explore the potential of aqueous two-phase systems (ATPS) for cell patterning.
To investigate the rheological properties and pattern formation of cell suspensions in ATPS.

Main Methods:

Utilized an aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) and dextran (DEX) solutions.
Formed capillary cell suspensions and water-in-water (DEX-in-PEG) capillary bridges.
Employed concentrated suspensions of yeast cells to form 3D patterns.

Main Results:

Demonstrated the successful 3D structuring of living cells using ATPS.
Observed enhanced rheological properties in concentrated yeast cell suspensions.
Achieved distinctive 3D patterns through capillary structured cell suspensions.

Conclusions:

The developed technique offers a facile approach to 3D cell structuring.
ATPS-based capillary cell suspensions show promise for advanced cell culturing.
Potential applications include instant tissue engineering and diverse biomedical investigations.