Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Transparent, Microfluidic Lab On A Chip For Multi-Modal Cell Culture Monitoring For Neurotoxicity Research.

IEEE transactions on nanobioscience·2026
Same author

Optical biosensing using particle diffusometry on thermoplastic microfluidic chips bonded using direct and indirect chip bonding methods.

Biomedical microdevices·2026
Same author

Development of an HPV 16 rapid test founded in user-centered design with primary care clinicians.

Analytical methods : advancing methods and applications·2025
Same author

Stakeholder considerations on acceptability and implementation of a novel rapid test for acute HIV infection: A qualitative study in Indiana.

PLOS global public health·2025
Same author

Antibody-Initiated Loop-Mediated Isothermal Nucleic Acid Amplification (ai-LAMP) as a New Biosensor for Antigen Detection.

bioRxiv : the preprint server for biology·2025
Same author

Protein Biomarkers Enable Sensitive and Specific Cervical Intraepithelial Neoplasia (CIN) II/III+ Detection: One Step Closer to Universal Cervical Cancer Screening.

Cancers·2025
Same journal

Dual Marker Co-Expressed Exosome-Based Liquid Biopsy Electrochemical Assay for Enhanced-Accuracy Diagnosis of Prostate Cancer.

ACS sensors·2026
Same journal

A Novel Strategy in Quartz‑Enhanced Spectroscopic Sensing Based on a Double‑Ended Quartz Tuning Fork.

ACS sensors·2026
Same journal

Paper Microfluidic Platform Using Multiplexed Isothermal Amplification and CRISPR/Cas12a for Aquatic Pathogen Detection.

ACS sensors·2026
Same journal

Strategic Design and Engineering of CRISPR/Cas-Powered Sensing Platforms for Enhanced Nucleic Acid Detection.

ACS sensors·2026
Same journal

Broad-Temperature Polymerase in Nucleic Acid Amplification-Based Diagnostics: From Thermal Precision to Dynamic Conditions.

ACS sensors·2026
Same journal

Fluidic Lipid-Bilayer-Enhanced Iontronic Nanopore: Machine-Learning-Driven Ultrasensitive MicroRNA Detection in Cancer Diagnostics.

ACS sensors·2026
See all related articles

Related Experiment Video

Updated: Jun 19, 2026

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy
13:08

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

Published on: October 7, 2010

16.5K

Real-Time Visualization of HIV-1 RNA Detection Using Loop-Mediated Isothermal Amplification-Enabled Particle

Dong Hoon Lee1,2, Emeka Nwanochie2, Katherine N Clayton3

  • 1School of Mechanical Engineering, Purdue University, West Lafayette, Indiana 47907, United States.

ACS Sensors
|October 8, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a new method using particle diffusometry (PD) for real-time monitoring of HIV-1 RNA amplification. This technique allows for rapid and accurate quantification of viral load, showing promise for disease management.

Keywords:
RT-LAMPdiagnosticsdiffusion coefficientfluorescenceisothermal amplificationparticle diffusometryreal time

More Related Videos

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection
07:24

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Published on: October 29, 2020

2.7K
Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform
07:15

Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform

Published on: May 18, 2021

3.1K

Related Experiment Videos

Last Updated: Jun 19, 2026

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy
13:08

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

Published on: October 7, 2010

16.5K
Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection
07:24

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Published on: October 29, 2020

2.7K
Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform
07:15

Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform

Published on: May 18, 2021

3.1K

Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Virology

Background:

  • Isothermal nucleic acid amplification tests (NAATs) like RT-LAMP offer rapid, real-time detection of viral pathogens.
  • Current methods for viral load monitoring can be complex and require specialized equipment.
  • There is a need for robust, field-deployable methods for accurate viral quantification.

Purpose of the Study:

  • To develop and validate a novel method for real-time, semiquantitative detection of HIV-1 RNA using RT-LAMP.
  • To utilize particle diffusometry (PD) for monitoring viral concentration during amplification.
  • To assess the speed, sensitivity, and accuracy of the developed method.

Main Methods:

  • Real-time monitoring of HIV-1 RNA RT-LAMP was performed using a novel particle diffusometry (PD) implementation.
  • Changes in the diffusion dynamics of 400 nm fluorescently labeled particles were tracked during the RT-LAMP reaction.
  • The method was tested for speed, sensitivity, and accuracy in quantifying HIV-1 RNA concentrations.

Main Results:

  • The developed PD-based RT-LAMP method achieved real-time measurement within 20 minutes.
  • Detection of HIV-1 RNA concentrations as low as 25 virus particles per μL was demonstrated.
  • A single-blind study showed semiquantitative detection with an absolute error margin of 10% for unknown HIV-1 RNA concentrations.

Conclusions:

  • Real-time particle diffusometry readout is a promising approach for quantifying HIV-1 RNA via RT-LAMP.
  • This method offers potential for rapid and accurate viral load monitoring in HIV and other chronic infections.
  • The technique's suitability for field use and simple instrumentation makes it a valuable diagnostic tool.