Types, synthesis pathways, purification, characterization, and agroecological physiological functions of microbial...

Exopolysaccharides (EPS), originating from various microbes, are essential bioproducts with widespread applications including packaging, biomedicine, wastewater treatment, cosmetics, agriculture, and food industries. Particularly, in the field of sustainable agriculture, microbial EPS have positive effects on plant growth and have gained considerable interest among agriculturists. However, few studies have elucidated the mechanisms of action of EPS in soil-microbe-plant interactions in agroecosystems. This review focuses on the sources and types of EPS, biosynthetic processes, factors affecting EPS yield, extraction and purification methods employed to produce microbial EPS, and structural characterization methods for EPS. Moreover, the agroecological physiological functions of microbial EPS with respect to promoting soil health (e.g., improving soil structure and fertility and repairing contaminated soil) and plant growth (e.g., plant growth and physiological metabolism under normal and stress conditions, such as salt, drought, heavy metals, and extreme temperatures) are critically highlighted. Furthermore, existing challenges and prospects for agricultural applications are discussed. This review demonstrates that the application of microbial EPS in agriculture provides a new type of green material for agricultural producers to improve soil quality, increase agricultural productivity, and provide new ideas for sustainable agricultural development.