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Updated: Jun 11, 2025
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Published on: June 8, 2020
Bone and periosteum protein analysis via tandem mass tag quantitative proteomics in pediatric patients with
Xinwu Wu1,2,3, Peisheng Chen1,2,3, Dianhua Huang1,2,3
1Department of Orthopedics, Fuzhou Second General Hospital, Fuzhou, China.
Insights
Pediatric osteomyelitis significantly impacts bone healing and callus formation. This proteomic study identified key proteins involved in inflammation and osteogenesis, offering new insights into bone repair mechanisms in children.
Area of Science:
- Orthopedics
- Pediatric Medicine
- Proteomics
- Molecular Biology
Background:
- Effective bone healing is critical for managing osteomyelitis, particularly after fracture fixation in pediatric patients.
- Understanding the mechanisms of extensive callus formation in pediatric osteomyelitis is essential for improving treatment outcomes.
Purpose of the Study:
- To elucidate the key molecular processes involved in callus formation during pediatric osteomyelitis.
- To analyze proteomic changes in bone and periosteum tissues from pediatric patients with osteomyelitis.
Main Methods:
- Tandem mass tag (TMT) quantitative proteomics was employed to analyze bone and periosteum samples from eight pediatric patients.
- Differential protein expression was analyzed using Gene Ontology (GO) annotation, GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, and protein-protein interaction networks.
Main Results:
- A total of 4737 proteins were identified, with 2224 differentially expressed proteins detected between bone and periosteum tissues.
- Ten protein genes in the bone group and nine in the periosteum group were associated with inflammation and osteogenesis.
- Specific proteins including CYBB, NAMPT, TIMP-1, RAF-1, RELA, and SGMS2 were highlighted for their potential roles in callus formation.
Conclusions:
- This study provides novel proteomic insights into the complex mechanisms of callus formation in pediatric osteomyelitis.
- Identified proteins offer potential therapeutic targets for enhancing bone healing in pediatric patients with osteomyelitis.
Abstract:
Bone healing is crucial in managing osteomyelitis after fracture fixation. Understanding the mechanism of extensive callus formation in pediatric osteomyelitis is highly important. This study aims to analyze bone and periosteum samples from pediatric patients to elucidate the essential processes involved in callus formation during osteomyelitis. The study included eight patients from our hospital: four with positive microbial culture who underwent osteomyelitis debridement and four who had osteotomy surgery as contral. We used tandem mass tag quantitative proteomics to investigate proteomic changes in bone and periosteum tissues obtained from these patients. Differential expression proteins were analyzed for their pathways through Gene Ontology (GO) annotation, GO enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and protein-protein interaction networks. A total of 4737 proteins were successfully identified. About 2224 differentially expressed proteins were detected in the bone tissues group and periosteum tissues group. Among the differentially expressed proteins, 10 protein genes in the bone group were associated with inflammation and osteogenesis, while in the periosteum group were nine. Cytochrome b-245, beta polypeptide (CYBB), nicotinamide phosphoribosyltransferase (NAMPT), tissue inhibitor of metalloproteinases 1 (TIMP-1), Raf-1 proto-oncogene, serine/threonine kinase (RAF-1), RELA proto-oncogene, NF-KB subunit (RELA), and sphingomyelin synthase 2 (SGMS2) may play an important role in callus formation in patients with osteomyelitis. This study provides novel clues for understanding callus formation in pediatric patients with osteomyelitis.
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