Related Experiment Video
Updated: Jun 11, 2025
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
Published on: August 18, 2018
Quality by design for mRNA platform purification based on continuous oligo-dT chromatography
Jixin Qu1, Adithya Nair1, George W Muir1
1School of Chemical, Materials and Biological Engineering, University of Sheffield, Sir Robert Hadfield Building, Mappin Street, Sheffield S1 3JD, UK.
A new continuous oligo-dT chromatography process enhances mRNA purification, reducing losses and improving resin efficiency. This method offers higher productivity and lower costs compared to traditional batch processing for mRNA therapeutics.
Area of Science:
- Biotechnology
- Chemical Engineering
- Pharmaceutical Manufacturing
Background:
- Oligo-deoxythymidine (oligo-dT) ligand-based affinity chromatography is crucial for mRNA purification.
- Conventional batch mode oligo-dT chromatography faces productivity limitations.
Purpose of the Study:
- To develop and optimize a continuous oligo-dT chromatography process for mRNA purification.
- To improve efficiency, reduce losses, and intensify the mRNA purification process.
- To establish a design space using the Quality by Design (QbD) framework.
Main Methods:
- Development of a continuous oligo-dT chromatography process.
- Optimization of process parameters (salt type, concentration, flow rate, load concentration) using a Quality by Design (QbD) framework.
- Evaluation of mRNA yield, integrity, and purity.
Main Results:
- Achieved >90% mRNA yield, >95% integrity, and >99% purity.
- Continuous chromatography demonstrated 5.75-fold higher productivity.
- Continuous chromatography offered an estimated 15% lower operating cost compared to batch mode.
Conclusions:
- The continuous oligo-dT chromatography process significantly enhances mRNA purification efficiency and cost-effectiveness.
- The QbD framework successfully established a design space for the continuous process.
- This intensified process is suitable for manufacturing mRNA-based vaccines and therapeutics.
More Related Videos
11:37Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
Published on: July 28, 2017
06:46Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
Published on: November 9, 2019