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Published on: June 7, 2024
Transcriptomic Communication between Nucleus and Mitochondria during the Browning Process of Lentinula edodes
Xiaoxia Song1, Meiyan Zhang1, Mingjie Chen1
1Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Cytoplasmic replacement impacts mushroom browning by altering nuclear and mitochondrial gene expression. This study reveals the internal antioxidant defense and external melanin production mechanisms influenced by cytoplasmic source and culture time.
Area of Science:
- * Mycology
- * Molecular Biology
- * Plant Pathology
Background:
- * Browning in *Lentinula edodes* (shiitake mushroom) is a significant factor affecting quality.
- * Previous studies have not fully elucidated the molecular mechanisms behind cytoplasmic replacement's effect on browning.
- * Understanding these mechanisms is crucial for improving mushroom cultivation and storage.
Purpose of the Study:
- * To investigate the molecular basis of cytoplasmic replacement's influence on *L. edodes* browning.
- * To identify key nuclear (N) and mitochondrial (M) genes and long noncoding RNAs (lncRNAs) involved in the browning process.
- * To explore the interplay between nuclear and mitochondrial genomes in regulating browning traits.
Main Methods:
- * Transcriptomic analysis of nuclear and mitochondrial mRNAs and lncRNAs.
- * Comparison of gene expression profiles in L808 and two cybrids (L808-A2, L808-B) at different culture times (80, 100, 120 days).
- * Analysis of gene expression changes related to oxidative stress, antioxidant machinery, and melanin production.
Main Results:
- * Cytoplasmic source and culture time significantly altered N, M gene, and lncRNA expression.
- * Cytoplasmic replacement modulated genes involved in internal browning (NADPH-mediated antioxidant defense against reactive oxygen species) and external browning (melanin aggregation).
- * Identified genes related to plasma membrane remodeling, extracellular enzymes, small molecules, and NADPH metabolism; reported novel nucleus-mitochondria communication via M-rps3.
Conclusions:
- * Cytoplasmic replacement significantly impacts *L. edodes* browning through altered gene expression.
- * The internal browning mechanism involves NADPH-dependent antioxidant protection against light-induced oxidative stress.
- * External browning is characterized by melanin accumulation, influenced by genes regulating membrane, enzymes, and metabolism; M-rps3 mediates nucleus-mitochondria communication.
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