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Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella
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Body temperature protein X-ray crystallography at 37 °C: a rhenium protein complex seeking a physiological condition
Francois J F Jacobs1, John R Helliwell2, Alice Brink1
1Department of Chemistry, University of the Free State, Nelson Mandela Drive, Bloemfontein 9301, South Africa.
Abstract:
The retention of the covalent binding of an organometalllic rhenium complex as a model for a technetium-99m imaging agent, to a protein at physiological body temperature 37 °C is described. Detailed structure comparisons are made to the related 100 K crystal structure. The generality of the need for this sort of analytical procedure for guiding ligand lead compound discovery is emphasised.
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