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Updated: Jun 11, 2025

Development of Compendium for Esophageal Squamous Cell Carcinoma
03:36

Development of Compendium for Esophageal Squamous Cell Carcinoma

Published on: April 12, 2024

385

EIF4A3-mediated oncogenic circRNA hsa_circ_0001165 advances esophageal squamous cell carcinoma progression through

Xun Zhang1,2, Yan Bian1,2, Qiuxin Li1,2

  • 1Department of Gastroenterology, Changhai Hospital, Naval Medical University, Shanghai, 200433, China.

Cell Biology and Toxicology
|October 9, 2024
PubMed

Insights

Circular RNAs (circRNAs) like hsa_circ_0001165 are elevated in esophageal squamous cell carcinoma (ESCC). This circRNA promotes ESCC progression by sponging miR-381-3p, making it a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Esophageal squamous cell carcinoma (ESCC) presents a significant clinical challenge with limited effective therapeutic targets.
  • Circular RNAs (circRNAs) play a critical role in the progression of various cancers, including ESCC.

Purpose of the Study:

  • To investigate the role of hsa_circ_0001165 in esophageal squamous cell carcinoma (ESCC).
  • To elucidate the molecular mechanisms underlying the function of hsa_circ_0001165 in ESCC progression.

Main Methods:

  • High-throughput sequencing to profile circRNA expression in ESCC tissues and cell lines.
  • In vitro and in vivo experiments to assess the functional impact of hsa_circ_0001165 knockdown.
  • Mechanism assays including dual-luciferase reporter gene, RNA Immunoprecipitation, and RNA-pulldown to determine molecular interactions.

Main Results:

  • hsa_circ_0001165 expression is significantly upregulated in ESCC tissues and cell lines, correlating with TNM staging.
  • Knockdown of hsa_circ_0001165 inhibited ESCC cell proliferation, invasion, and migration in vitro and suppressed tumor growth in vivo.
  • hsa_circ_0001165 acts as a molecular sponge for miR-381-3p, leading to increased TNS3 expression and promoting aggressive ESCC phenotypes.

Conclusions:

  • Upregulated hsa_circ_0001165 promotes ESCC progression by modulating the miR-381-3p/TNS3 axis.
  • hsa_circ_0001165 represents a promising biomarker and potential therapeutic target for esophageal squamous cell carcinoma.
Keywords:
Esophageal Squamous Cell CarcinomaHsa_circ_0001165MiR-381-3pTNS3

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