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Updated: Jun 12, 2025
Isolating Lymphocytes from the Mouse Small Intestinal Immune System
Published on: February 28, 2018
Protocol for isolating innate lymphoid cells from murine lamina propria of the small intestine
1Department of Pathogenic Biology and Immunology, School of Medicine, Southeast University, Nanjing, Jiangsu 210009, China.
Abstract:
Innate lymphoid cells (ILCs) in the lamina propria of the small intestine (siLP) are essential for maintaining intestinal immune homeostasis; however, their isolation remains challenging. Here, we present a protocol for the rapid isolation of siLP ILCs. We describe steps for small intestine collection and trimming, epithelial cell dissociation, lamina propria digestion, and ILC analysis by flow cytometry. For complete details on the use and execution of this protocol, please refer to Zheng et al.1.
