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Updated: Jun 11, 2025

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

TWINKLE: An open-source two-photon microscope for teaching and research.

Manuel Schottdorf^1,2, P Dylan Rich¹, E Mika Diamanti¹

¹Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA.

Biorxiv : the Preprint Server for Biology

|October 10, 2024

Summary

This summary is machine-generated.

We developed "Twinkle," an open-source, easy-to-use two-photon microscope for neuroscience research and education. This high-performance system simplifies training and customization, making advanced imaging accessible.

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Area of Science:

Neuroscience
Optical Microscopy
Open Science Instrumentation

Background:

Commercial and complex homemade two-photon microscopes hinder customization, troubleshooting, and training.
Integrated systems present significant barriers for educational and research accessibility.

Purpose of the Study:

Introduce "Twinkle," an open, high-performance, and user-friendly two-photon microscope.
Facilitate both neuroscience research and educational training through accessible instrumentation.

Main Methods:

Designed a fully open two-photon microscope system named "Twinkle."
Integrated a large field of view (> 1 mm) with a 3 mm working distance objective and 2-inch optics.
Utilized GaAsP photomultiplier tubes for enhanced fluorescence signal detection.

Main Results:

Demonstrated effective use of "Twinkle" as a teaching tool in multiple workshops.
Showcased scientific use cases highlighting the system's research capabilities.
Achieved a > 1 mm field of view with optimized optical components and sensitive detectors.

Conclusions:

"Twinkle" offers a high-performing, easy-to-set-up alternative to complex microscopy systems.
The open-source nature promotes customization, troubleshooting, and broader adoption in education and research.
This work contributes to the growing field of accessible open scientific instrumentation.