TWINKLE: An open-source two-photon microscope for teaching and research

Area of Science:

• Neuroscience
• Optical Microscopy
• Open Science Instrumentation

Background:

• Commercial and complex homemade two-photon microscopes hinder customization, troubleshooting, and training.
• Integrated systems present significant barriers for educational and research accessibility.

Purpose of the Study:

• Introduce "Twinkle," an open, high-performance, and user-friendly two-photon microscope.
• Facilitate both neuroscience research and educational training through accessible instrumentation.

Main Methods:

• Designed a fully open two-photon microscope system named "Twinkle."
• Integrated a large field of view (> 1 mm) with a 3 mm working distance objective and 2-inch optics.
• Utilized GaAsP photomultiplier tubes for enhanced fluorescence signal detection.

Main Results:

• Demonstrated effective use of "Twinkle" as a teaching tool in multiple workshops.
• Showcased scientific use cases highlighting the system's research capabilities.
• Achieved a > 1 mm field of view with optimized optical components and sensitive detectors.

Conclusions:

• "Twinkle" offers a high-performing, easy-to-set-up alternative to complex microscopy systems.
• The open-source nature promotes customization, troubleshooting, and broader adoption in education and research.
• This work contributes to the growing field of accessible open scientific instrumentation.