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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

Updated: Jun 11, 2025

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

Published on: October 4, 2010

An open-source combined atomic force microscope and optical microscope for mechanobiology studies.

Daniel Delgado¹, Sarah Desroches^1,2, Gia Kang¹

¹Department of Mechanical and Aerospace Engineering, Carleton University, 1125 Colonel by Drive, Ottawa, Ontario, Canada, K1S 5B6.

|October 10, 2024

Summary

This summary is machine-generated.

This study introduces an affordable, user-friendly Atomic Force Microscopy (AFM) system. It measures cell mechanics and mechanosensitive responses, making advanced biophysical techniques more accessible.

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Last Updated: Jun 11, 2025

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

Published on: October 4, 2010

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

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Published on: June 27, 2013

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Area of Science:

Biophysics
Cell Biology
Materials Science

Background:

Atomic Force Microscopy (AFM) is crucial for measuring biological sample mechanical properties.
High cost and expertise required for AFM limit its widespread adoption by non-experts.
Understanding cell mechanics is vital for physiology and disease research.

Purpose of the Study:

To develop an accessible and cost-effective AFM-based system for measuring cell mechanical properties.
To integrate a fluorescence microscope for characterizing mechanosensitive responses.
To facilitate interdisciplinary research into cell mechanics and disease.

Main Methods:

Designed a novel system combining AFM with a custom inverted fluorescence microscope.
Focused on ease of use and reduced setup costs.
Utilized AFM for precise mechanical measurements of cellular structures.

Main Results:

The developed system effectively measures cell mechanical properties.
The integrated fluorescence microscopy allows for simultaneous characterization of mechanosensitive responses.
The system's design addresses the accessibility barriers of traditional AFM.

Conclusions:

The new AFM system lowers the barrier for entry into cell mechanics research.
This technology can drive new discoveries in the role of cell mechanics in health and disease.
Promotes interdisciplinary collaboration by making advanced techniques more accessible.